Applications - Specific hydrolysis of oligosaccharides with type I chain and production of lacto-N-biose (Galβ1-3GlcNAc)
Source Streptomyces sp. 142
Storage The enzyme solution of 1 mU/mL is stable for at least 12 months at below 4°C.
Properties - Concentration: 1 µU/µL
- Molecular weight: 60 kDa (SDS-PAGE)
- Km (substrate): 6.80 µM Pyridylamino (PA)-lacto-N-tetraose); 38.9 µM (PA-oligosaccharide c)
- Optimum pH: pH 5.5 (sodium acetate buffer)
- Range of pH stability: pH 4.0–10 (4°C, 16 hrs.)
Form Solution in 50 mM sodium acetate buffer, pH 5.5, containing 0.05% Brij 58
Definition of Activity One unit is the amount of enzyme required to hydrolyze 1 µmol of PA-lacto-N-tetraose in 1 minute at 37°C, pH 5.5.
References - Sano, M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8512-6.
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