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Products >  Glycobiology >  Glycobiology_Enzymes_and_Reagents >  Lacto-N-Biosidase

Lacto-N-Biosidase

Lacto-N-Biosidase may be used during glycoprotein analysis to better study the structure and function of glycoprotein and glycolipid sugar chains. This product can be used for specific oligosaccharide hydrolysis of type-I chain oligosaccharides, producing lacto-N-biose (Gal β 1-3 GlcNAc) as a byproduct, but not oligosaccharide hydrolysis of type-II chain oligosaccharides. As a result, Lacto-N-Biosidase can be used to distinguish type-I versus type-II glycoprotein as well as glycolipid sugar chains. When used in conjunction with α -1,3/4-Fucosidase, Lacto-N-Biosidase can also help distinguish Sialyl-Lewis x and Sialyl-Lewis a structures.

One vial of Lacto-N-Biosidase (100 µU) allows 50–100 enzymatic digestions of up to 10 pmol of sugar chains.

  At-A-Glance   Documents   Images & Data

Applications

  • Specific hydrolysis of oligosaccharides with type-I sugar chains and production of byproduct lacto-N-biose (Gal β 1-3 GlcNAc)

Source

Streptomyces sp. 142

Storage

-20°C

Properties

  • Concentration: 1 µU/µL
  • Molecular weight: 60 kDa (SDS-PAGE)
  • Km (substrate): 6.80 µM (Pyridylamino (PA)-lacto-N-tetraose); 38.9 µM (PA-oligosaccharide c)
  • Optimum pH: pH 5.5 (sodium citrate buffer, sodium phosphate buffer)
  • Range of pH stability: pH 4.0–10 (4°C, 16 hrs.)

Form

Solution in 50 mM sodium acetate buffer, pH 5.5, containing 0.05% Brij-58

Definition of Activity

One unit is the amount of enzyme required to hydrolyze 1 µmol of PA-lacto-N-tetraose in 1 minute at 37°C, pH 5.5.

References

  1. Sano, M., Hayakawa, K. and Kato, I. (1992) Proc. Natl. Acad. Sci. USA 89, 8512-8516
  2. Sano, M., Hayakawa, K. and Kato, I. (1993) J. Biol. Chem. 268, 18560-18566
  3. Takasaki, S. and Kobata, A.(1986) Biochemistry 25, 5709-5715
  4. Townsend, R. R., Hardy, M. R., Wong, T. C. and Lee, Y. C. (1986) Biochemistry 25, 5716-5725
  5. Stroud, M. R., Levery, S. B., Nudelman, E. D., Salyan, M. E. K., Towell, J. A., Roberts, C. E., Watanabe, M. and Hakomori, S. (1991) J. Biol. Chem. 266, 8439-8446
  6. Sano, M., Hayakawa, K. and Kato, I. (1992) J. Biol. Chem. 267,1522 -1527.



 
 
Products
Cat. # Product Contents Size Price Units Select
4456 Lacto-N-biosidase 100 uU $495.00
 

 

 
 

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