Takara
login | register | order now | view cart | feedback
Products
Glycobiology


Products >  Glycobiology >  Glycobiology_Enzymes_and_Reagents >  Glycopeptidase_F

PNGase F (Glycopeptidase F)

Glycopeptidase F, also known as PNGase F or Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase, removes N-glycan groups from glycoproteins and glycopeptides. Deglycosylation enables proteomic analysis, including MALDI-TOF mass spectrometry. PNGase F is often used to determine if proteins contain glycosylated peptides and if deglycosylation affects a protein's enzymatic activity. Removal of N-linked glycans prior to MALDI-TOF and other mass spectrometry procedures may also be performed to reduce protein heterogeneity and thereby facilitate proteomic analysis. PNGase F is one of the most widely used enzymes for deglycosylation of glycoproteins and glyopeptides.

At-A-Glance Documents

Applications

  • Determination of protein and/or peptide glycosylation level
  • Analysis of N-glycan functional roles
  • Reduction of protein heterogeneity
  • MALDI-TOF or other mass spectrometry

Components

Denature buffer : 1% SDS / 1M Tris-HCl (pH8.6) 500 μl
Native buffer : 1M Tris-HCl (pH 8.6) 500 μl
Stabilized solution : 5% Nonidet P-40 500 μl
Control Glycoprotein : 10 mg/ml Bovine Fetuin 10 μl

Source

Recombinant Escherichia coli encoding PNGase F.

Storage

-20ºC

Substrate Specificity

Specific cleavage of N-glycan (GluNAc-Asn) bonds in glycoproteins and glycopeptides.

PNGase F (Glycopeptidase F) will not cleave N-glycan groups when the innermost GlcNAc residue is linked to an alpha1-3 Fucose residue.  This modification is found in plant glycoproteins as well as some insect glycoproteins.

Definition of activity

One unit is the amount of enzyme required to hydrolyze 1 μmol of dansyl fetuin glycopeptide within 1 minitue at 37℃ at pH 8.6.

Properties

  • Molecular weight : approx. 36.5 kDa (SDS-PAGE)

    (Note) Depending on proteolytic activity of host bacteria, recombinant PNGase F may have 15 or 18 additional animo acid residues attached to its N-terminus. However, PNGase F glycosylase activity has been confirmed to be identical to that of native PNGase F via experiments using RNase B and Fetuin as substrates.

  • Optimum pH: 8.6



 
 
Products
Cat. # Product Package Size Price # of Units Select
4450 Glycopeptidase F (Peptide: N-glycosidase F) 25 mU $244.00
 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions