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Products >  Glycobiology >  Glycobiology_Enzymes_and_Reagents >  Cloned_alpha2-3-Sialidase

Cloned alpha 2,3-Sialidase

This enzyme has a 260-fold kinetic preference for the alpha 2,3 sialyl linkage. The enzyme efficiently cleaves sialyl groups from glycoproteins and glycolipids. However, the enzyme may not release sialic acid from O-linked oligosaccharides on glycoproteins.

At-A-Glance Documents Images & Data

Features

  • This sialidase has a 260-fold kinetic preference for the alpha 2,3 sialyl linkage
  • The enzyme efficiently cleaves sialyl groups from glycoproteins and glycolipids
  • The enzyme may not release sialic acid from O-linked oligosaccharides on glycoproteins

Source

E. coli (cloned from Salmonella typhimurium LT2)

Storage

4°C

Properties

  • Systematic Name: Acylneuraminyl hydrolase (E.C. 3.2.1.18)
  • Molecular weight: 41.3 kDa (SDS-PAGE)
  • Km (substrate):
    • 0.25 mM (4MUNeuAc)
    • 1.52 mM (3'-Sialyliactose)
    • 2.66 mM (PA-3'-sialyllactose)
  • Inhibitors:
    • 2-deoxy-2,3-didehydro-N-acetyl-neuraminic acid
    • Ki = 0.38 mM
  • Optimum pH:
    • pH 5.5–7.0 (Tris-maleate buffer)
    • pH 5.5–6.0 (potassium phosphate buffer, sodium acetate buffer, Tris-HCl buffer)

Form

Solution containing 50 mM potassium phosphate (pH6.8), 0.1 M sodium chloride, and 0.02% sodium azide.

References

  1. Hoyer, L.L. (1992) Mol. Microbiol. 6 873-884.
  2. Hoyer, L.L. (1991) J. Biochem. 110:462-7.

Definition of Activity

One unit is the amount of enzyme required to hydrolyze 1 µmol of 3'-sialyllactose in 1 minute at 37°C, pH 5.5.




 
 
Products
Cat. # Product Package Size Price # of Units Select
4455 Cloned alpha 2,3-Sialidase 5 Units $360.00
 

 



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