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Epigenetics


Products >  Epigenetics >  Histone_Modification_Analysis >  Anti-Histone_Antibodies

Epigenetic Analysis: Anti-Histone Antibodies

The following mouse monoclonal histone antibodies are suitable for epigenetic analysis of histone modifications such as acetylation and phosphorylation. These antibodies may be used for chromatin immunoprecipitation (ChIP) assays as well as immunoprecipitation and immunoblotting applications.

At-A-Glance Documents Images & Data

Features

  • Host: Mouse
  • Antigen: Histone H3 antibodies were generated via a 13-amino acid peptide on N-terminal human histone H3.1. The Anti-Phospho Histone H2B (Ser14) antibody (MA251B) was generated via a 19-amino acid peptide surrounding Ser14 on human histone H2B.
  • Buffer: PBS + 0.05% sodium azide
  • Immunoprecipitation: 1–5 µg/5 µL Sepharose
  • Immunostaining: 0.5–1 µg/mL
  • Immunoblot: 0.5–1 µg/mL

Applications

  • Chromatin immunoprecipitation 
  • Epigenetic analysis of histone modifications

Source

Serum-free culture medium supernatant

Storage

Store below –20°C

Notes

Mouse IgG binds weakly with protein A and protein G. When performing immunoprecipitation with these antibodies, use beads that bind anti-mouse IgG antibodies strongly, such as anti-mouse IgG Sepharose.

Concentration

1 mg/mL

Reference

  1. Kimura, H., et al. (2008) Cell Struct. Funct. 33(1):61-73.
  2. Kim H.-G., et al. (2008) Cancer Res. 68:2538-2547.



 
 
Products
Cat. # Product Package Size Price # of Units Select
MA309B Anti-Acetyl Histone H3 (Lys27), mouse monoclonal antibody 100 uL $336.00
MA305B Anti-Acetyl Histone H3 (Lys9), mouse monoclonal antibody 100 uL $336.00
MA310B Anti-Acetyl Histone H3 (Lys9/27), mouse monoclonal antibody 100 uL $336.00
MA332B Anti-Dimethyl Histone H3 (Lys36), Mouse Monoclonal Antibody 100 uL $336.00
MA307B Anti-Dimethyl Histone H3 (Lys9), mouse monoclonal antibody 100 uL $336.00
MA301B Anti-Histone H3 (mouse), Monoclonal 100 uL $336.00
MA321B Anti-Monomethyl Histone H3 (Lys27), Mouse Monoclonal Antibody 100 uL $336.00
MA331B Anti-Monomethyl Histone H3 (Lys36), Mouse Monoclonal Antibody 100 uL $336.00
MA302B Anti-Monomethyl Histone H3 (Lys4), mouse monoclonal antibody 100 uL $336.00
MA306B Anti-Monomethyl Histone H3 (Lys9), mouse monoclonal antibody 100 uL $336.00
MA251B Anti-Phospho Histone H2B (Ser14), Mouse Monoclonal Antibody 100 uL $336.00
MA312B Anti-Phospho Histone H3 (Ser10), mouse monoclonal antibody 100 uL $336.00
MA323B Anti-Trimethyl Histone H3 (Lys27), Mouse Monoclonal Antibody 100 uL $336.00
MA333B Anti-Trimethyl Histone H3 (Lys36), Mouse Monoclonal Antibody 100 uL $336.00
MA304B Anti-Trimethyl Histone H3 (Lys4), mouse monoclonal antibody 100 uL $336.00
MA308B Anti-Trimethyl Histone H3 (Lys9), mouse monoclonal antibody 100 uL $336.00
MA303B Anti-dimethyl Histone H3 (Lys4), mouse monoclonal antibody 100 uL $336.00
 

 



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