- Addition of glucose to 5-hydroxymethylcytosine (to create β-glucosyl-5-hydroxymethylcytosine) in mammalian or plant genomic DNA.
- Detection of 5-hydroxymethylcytosine when coupled with digestion with a restriction enzyme that is sensitive to β-glucosyl-5-hydroxymethylcytosine.
|10X T4-BGT Buffer||120 µl|
|50X UDP-Glucose||25 µl|
Escherichia coli carrying a plasmid that enables expression of β-glucosyltransferase from bacteriophage T4.
Nuclease activity is not detected after incubation of 1 µg of DNA (pBR322 and λ-HindIII digest) with 50 units of this enzyme for 4 hours at 37°C as assessed by agarose gel electrophoresis.