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Products >  Epigenetics >  DNA_Preparation >  hydroxymethylcytosine_T4-beta-glucosyltransferase

Hydroxymethylcytosine Analysis: T4 β-glucosyltransferase

5-hydroxymethylcytosine (5-hmC) is an epigenetic modification (methylated cytosine) that is thought to be a derivative of 5-methylcytosine (5-mC). T4 β-glucosyltransferase catalyzes the transfer of glucose from uridine diphosphoglucose (UDP-Glucose) to the hydroxymethyl group 5-hmC, but does not modify 5-mC. This modifying enzyme can be used to differentiate these types of cytosine methylation. Specifically, after T4 β-glucosyltransferase treatment, restriction enzyme (e.g., MspI/HpaII) digestion or immunodetection can be used identify β-glucosyl-5-hydroxymethylcytosine (ß-glu-5hmC) at particular loci, or genome-wide analyses can be accomplished by selective pull-down of β-glu-5hmC.

At-A-Glance Documents

Applications:

  • Addition of glucose to 5-hydroxymethylcytosine (to create β-glucosyl-5-hydroxymethylcytosine) in mammalian or plant genomic DNA.
  • Detection of 5-hydroxymethylcytosine when coupled with digestion with a restriction enzyme that is sensitive to β-glucosyl-5-hydroxymethylcytosine.

Components:

10X T4-BGT Buffer120 µl
50X UDP-Glucose25 µl

Source:

Escherichia coli carrying a plasmid that enables expression of β-glucosyltransferase from bacteriophage T4.

Purity:

Nuclease activity is not detected after incubation of 1 µg of DNA (pBR322 and λ-HindIII digest) with 50 units of this enzyme for 4 hours at 37°C as assessed by agarose gel electrophoresis.

Storage:

-20°C




 
 
Products
Cat. # Product Package Size Price # of Units Select
2350A T4-Beta-glucosyltransferase 500 Units $77.00
2350B T4-Beta-glucosyltransferase 1,500 Units $204.00
 

 



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