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Products >  Epigenetics >  DNA_Preparation >  MethylEasy_DNA_Bisulphite_Modification_Kits

DNA Methylation Analysis: MethylEasy DNA Bisulphite Modification Kits

The MethylEasy DNA Bisulphite Modification Kit provides a simple, sensitive and quick DNA methylation analysis method that requires no DNA pre-treatment and uses as little as 50 pg of starting material. MethylEasy sodium bisulphite conversion technology results in virtually no loss of DNA, improved sensitivity, higher amplification efficiency, longer fragment generation and increased stability of the template DNA.

The MethylEasy Xceed system addresses the limitations of conventional DNA bisulphite modification treatments. These limitations include the loss of 84%–96% of starting DNA, required pre-treatment with restriction endonucleases and DNA agarose analysis combined with multiple tube changes and precipitations.

We recommend using TaKaRa Taq DNA Polymerase Hot Start with the MethylEasy and MethylEasy Xceed products.

At-A-Glance Documents Images & Data Resources

Features

  • Easy to use: No DNA pre-treatment necessary, easy column purification.
  • More conversions per kit: Sufficient reagent for up to 40 conversion reactions.
  • Quicker reaction time: Processing time is 90 min. from wild type to fully modified DNA.
  • High sensitivity: Start from as little as 50 pg of DNA (~8 mammalian cell equivalents).
  • Excellent DNA preservation: Reduced DNA degradation (> 90%) for true analysis of methylated cytosine.
  • Improved DNA conversion: No evidence of non-conversion found in sequence analysis.
  • Internal controls are included for experimental outcome confidence and troubleshooting.

Applications

  • Detection and quantification of transgenes inserted into transgenic mouse genome.

Components

ME001 (MethylEasy)

Reagent #11 x 5.2 mL
Reagent #21 x 2 g
Reagent #31 x 3.0 mL
Reagent #41 x 25 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Primers #3A and 3B2 x 40 µL
Microcentrifuge tubes25 x 2 mL tubes

ME002 (MethylEasy Xceed)

Reagent #12 x 5.0 mL
Reagent #22 x 3.3 g
Reagent #31 x 10.6 mL
Reagent #41 x 4.6 mL
Reagent #51 x 5.0 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Primers #3A and 3B2 x 40 µL
Purification Columns40 columns
Collection Tubes40 x 2 mL tubes

MEHT002 (MethylEasy High Throughput Centrifuge Method)

Reagent #11 x 20.8 mL
Reagent #21 x 8 g
Reagent #31 x 25 mL
Reagent #41 x 7 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Sample #3A and 3B2 x 40 µL
Conversion plate1 x 96 well
Purification plate2 x 96 well
Wash plate2 x 96 well
Elution plate2 x 96 well
Clear plate sealing film for Purification plate2 x film
Sealing caps for Conversion plate12 x 8 strips

MEHT003 (MethylEasy High Throughput Vacuum Method)

Reagent #11 x 20.8 mL
Reagent #21 x 8 g
Reagent #31 x 25 mL
Reagent #41 x 7 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Sample #3A and 3B2 x 40 µL
Conversion plate1 x 96 well
Purification plate2 x 96 well
Wash plate2 x 96 well
Elution plate in rack2 x 96 well
Clear plate sealing film for Elution plate1 x film
Sealing caps for Conversion plate12 x 8 strips
Sealing caps for Elution plate8 x 12 strips

Storage

–20°C




 
 
Products
Cat. # Product Package Size Price # of Units Select
ME001 MethylEasy™ DNA Bisulphite Modification Kit 25 Rxns $311.00
MEHT002 MethylEasy™ HighThroughput for Centrifuge 96 Well Plate $987.00
MEHT003 MethylEasy™ HighThroughput for Vacuum 96 Well Plate $987.00
ME002 MethylEasy™ Xceed Rapid DNA Bisulphite Modification Kit 40 Rxns $207.00
 

 

Recommended Products
Offers Cat. # Product Package Size Price License # of Units Select
R007A TaKaRa Taq™ DNA Polymerase Hot Start Version 250 Units $138.00 License Statements
 

 



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