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Products >  Epigenetics >  DNA_Preparation >  MethylEasy_DNA_Bisulphite_Modification_Kits

MethylEasy DNA Bisulphite Modification Kits

MethylEasy Xceed is a simple, sensitive and quick modification method that requires no DNA pre-treatment and uses as little as 50 pg of starting material. MethylEasy "loss-less" sodium bisulphite technology results in virtually no loss of DNA, improved sensitivity, higher amplification efficiency, longer fragment generation and increased stability of the template DNA.

MethylEasy Xceed addresses the the limitations of conventional bisulphite treatments. These limitations include the loss of 84%–96% of the starting DNA, requirement for pre-treatment with restriction endonuclease digests and the embedding of the DNA in agarose or multiple tube changes and precipitations.

Use TaKaRa Taq HS with the MethylEasy products.

At-A-Glance Documents Resources

Features

  • Easy to use: no DNA pre-treatment necessary and easy column purification
  • Increased conversions per kit: enough reagent for 40 conversions
  • Faster reactions: processing time is 90 mins. total from wild type to fully modified DNA
  • High sensitivity: start from as little as 50 pg of DNA (approx. 8 mammalian cell equivalents)
  • Excellent DNA preservation: reduce DNA degradation by more than 90% to obtain truly representative analysis of methylated cytosines
  • Better conversion: no evidence of DNA non-conversion found in sequencing analysis
  • Internal controls: included for absolute reaction confidence and product support

Applications

  • Detection and quantification of transgene inserted in transgenic mouse genome

Components

ME001 (MethylEasy)

Reagent #11 x 5.2 mL
Reagent #21 x 2 g
Reagent #31 x 3.0 mL
Reagent #41 x 25 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Primers #3A and 3B2 x 40 µL
Microcentrifuge tubes25 x 2 mL tubes

ME002 (MethylEasy Xceed)

Reagent #12 x 5.0 mL
Reagent #22 x 3.3 g
Reagent #31 x 10.6 mL
Reagent #41 x 4.6 mL
Reagent #51 x 5.0 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Primers #3A and 3B2 x 40 µL
Purification Columns40 columns
Collection Tubes40 x 2 mL tubes

MEHT002 (MethylEasy High Throughput Centrifuge Method)

Reagent #11 x 20.8 mL
Reagent #21 x 8 g
Reagent #31 x 25 mL
Reagent #41 x 7 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Sample #3A and 3B2 x 40 µL
Conversion plate1 x 96 well
Purification plate2 x 96 well
Wash plate2 x 96 well
Elution plate2 x 96 well
Clear plate sealing film for Purification plate2 x film
Sealing caps for Conversion plate12 x 8 strips

MEHT003 (MethylEasy High Throughput Vacuum Method)

Reagent #11 x 20.8 mL
Reagent #21 x 8 g
Reagent #31 x 25 mL
Reagent #41 x 7 mL
Control Sample #11 x 40 µL
Control Sample #21 x 20 µL
Control Sample #3A and 3B2 x 40 µL
Conversion plate1 x 96 well
Purification plate2 x 96 well
Wash plate2 x 96 well
Elution plate in rack2 x 96 well
Clear plate sealing film for Elution plate1 x film
Sealing caps for Conversion plate12 x 8 strips
Sealing caps for Elution plate8 x 12 strips

Storage

–20°C




 
 
Products
Cat. # Product Package Size Price # of Units Select
ME001 MethylEasy™ DNA Bisulphite Modification Kit 25 Rxns $311.00
MEHT002 MethylEasy™ HighThroughput for Centrifuge 96 Well Plate $987.00
MEHT003 MethylEasy™ HighThroughput for Vacuum 96 Well Plate $987.00
ME002 MethylEasy™ Xceed Rapid DNA Bisulphite Modification Kit 40 Rxns $207.00
 

 

Recommended Products
Cat. # Product Package Size Price License # of Units Select
R007A TaKaRa Taq™ DNA Polymerase Hot Start Version 250 Units $134.00 License Statements
 

 



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