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Methylation Sensitive Restriction Enzymes

Methylation Sensitive Restriction Enzymes

Methylation Sensitive Restriction Enzymes (MSREs) play a role in the analysis of methylated DNA. MSREs are used to analyze the methylation status of cytosine residues in CpG sequences. These restriction enzymes, as their name implies, are not able to cleave methylated-cytosine residues, leaving methylated DNA intact. MSREs cleave DNA at specific unmethylated-cytosine residues. DNA is amplified by PCR following digestion. Because unmethylated DNA is digested by MSREs, the only amplification products detected are methylated DNA. It is imperative to fully digest the DNA before PCR amplification to generate accurate data.

The table below provides a summary list of methylation sensitive restriction enzymes (MSREs) that may be useful for mammalian epigenetics analyses when used with CpG methylase.

Restriction Enzyme Recognition Sequence Catalog Number
Aat II GACGT↓C 1112A/B
Acc II CG↓CG 1002A/B
Aor13H I T↓CCGGA 1224A/B
Aor51H I AGC↓GCT 1118A/B

BspT104 I TT↓CGAA 1225A/B
BssH II G↓CGCGC 1119A/B

Cfr10 I R↓CCGGY 1120A/B
Cla I AT↓CGAT 1034A/B/AH/BH
Cpo I CG↓GWCCG 1035A/B
Eco52 I C↓GGCCG 1039A/B
Hae II RGCGC↓Y 1052A/B
Hap II C↓CGG 1053A/B/AH/BH
Hha I GCG↓C 1056A/B
Mlu I A↓CGCGT 1071A/B/AH/BH
Nae I GCC↓GGC 1155A/B
Not I GC↓GGCCGC 1166A/B/BH
Nru I TCG↓CGA 1168A/B
Nsb I TGC↓GCA 1226A/B
PmaC I CAC↓GTG 1177A/B
Psp1406 I AA↓CGTT 1108A/B
Pvu I CGAT↓CG 1242A/B
Sac II CCGC↓GG 1079A/B
Sal I G↓TCGAC 1080A/B/AH/BH
Sma I CCC↓GGG 1085A/B/AH/BH
SnaB I TAC↓GTA 1245A/B
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