EpiTaq HS (for bisulfite-treated DNA) is a DNA polymerase optimized for PCR amplifications using bisulfite-treated DNA containing uracil as template. PCR reactions with bisulfite-treated DNA often pose particular technical challenges for conventional enzymes. TaKaRa EpiTaq HS and the reagents contained in this kit, however, allow adjustment of magnesium and dNTP concentrations to achieve optimal amplification efficiencies and specificities, facilitating excellent amplifications even for targets that previously were impossible to amplify.
EpiTaq HS is also a hot-start PCR enzyme with an anti-Taq antibody. Prior to high temperature denaturation, the anti-Taq antibody suppresses polymerase activity, thereby preventing non-specific amplifications as a result of mispriming or primer dimer formation before starting PCR. This allows the use of conventional PCR conditions without requiring a special denaturation step.
