This kit is designed for the preparation of nucleosomes from cultured mammalian cells. DNA can be extracted from the prepared nucleosome and analyzed using a real-time PCR assay or high-speed sequencing to map nucleosome positions.
Nucleosomes are the constituent units of chromatin in eukaryotic nuclei, consisting of genomic DNA wrapped around a core histone complex. Nucleosomes are not spaced at regular intervals on genomic DNA. Their positions are known to be closely related to chromatin structure and transcriptional regulation. In epigenetics research, which is an important area in the study of the regulation of gene expression, nucleosome positioning has become a subject of intense interest. In genes activated for transcription, the promoter region is free of nucleosomes, allowing the binding of transcriptional initiation factors. However, DNA methylation in the promoter region leads to formation of nucleosomes, preventing the binding of transcription factors and allowing transcriptional suppression.
