EpiScope MSP Kit contains a PCR reagent designed exclusively for Methylation Specific PCR (MSP). A specific enzyme combined with an optimized buffer allows MSP analyses using a bisulfite-treated DNA template containing uracil. This kit provides a higher amplification efficiency and greatly improved ability to differentiate methylated/unmethylated DNA compared with traditional PCR reagents. The reaction system has been optimized for real-time monitoring using SYBR Green I1, and makes it possible to perform both real-time PCR and endpoint detection under the same reaction conditions.
Principle of MSP
The first step in methlylation specific PCR is to identify a nucleotide region whose sequence is subject to change by bisulfite treatment depending on the methylation status of the CpG sequence. Next, design two primers for this region; one for methylated CpG DNA (M primer), and the other for unmethylated DNA (UM primer). The last step is PCR amplification.
Note: 1Takara Bio is under a license agreement with Molecular Probes Inc. for the use of SYBR Green I as a reagent for research purposes. SYBR is a registered trademark of Molecular Probes Inc.
