Principle of MSP
Methlylation specific PCR identifies nucleotide regions in which cytosines have been altered to uracils following bisulfite treatment of the DNA template. These alterations are a function of the DNA template's CpG methylation status; methylated cytosines remain unaltered while the unmethylated cytosines are altered to uracils. Two primers are designed to detect these unaltered or altered regions, the methylated CpG DNA (M primer) and the unmethylated CpG DNA (UM primer), respectively. The last step involves methylation specific PCR.