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Products >  Epigenetics >  DNA_Methylation_Analysis >  Control_DNA

Control DNA for DNA Methylation Analysis

EpiScope Methylated HeLa gDNA is genomic DNA that is purified from human HeLa cells and highly methylated using CpG methylase. EpiScope Methylated HCT116 gDNA is genomic DNA purified from human HCT116 cells that is highly methylated using CpG methylase. Both are used as positive controls for DNA methylation analysis methods such as bisulfite sequencing, Combined Bisulfite Restriction Analysis (COBRA), Methylated CpG Island Recovery Assay (MIRA) and Methylation Specific PCR (MSP). Episcope Unmethylated HCT116 DKO gDNA is genomic DNA purified from the DNMT DKO (double knock-out) human HCT116 cell line, which is genetically lacking in both methyltransferases DNMT1 (DNA methyltransferase 1) and DNMT3B (DNA methytransferase 3B). The methylation level of the genomic DNA from DNMT DKO HCT116 cell line is much lower (under 5%) than wild type genomic DNA. Therefore, this DNA can be used as a negative (un- or hypo-methylated) control in DNA methylation analyses including bisulfite sequencing, COBRA, MIRA and MSP.

DNA methylation analysis is a field of epigenetics research that has been gaining attention in recent years. Methylation of CpG islands in the promoter region of a gene may influence that gene's transcriptional regulation. Bisulfite sequencing, Methylation Specific PCR (MSP), Combined Bisulfite Restriction Analysis (COBRA) and Methylated CpG Island Recovery Assay (MIRA) are all commonly used to analyze methylated DNA.

At-A-Glance Documents Images & Data Resources

Applications

Can be used as a control for the following DNA methylation analysis techniques:

  • Bisulfite Sequencing
  • Combined Bisulfite Restriction Analysis (COBRA)
  • Methylated CpG Island Recovery Assay (MIRA)
  • Methylation Specific PCR (MSP)

Source

EpiScope Methylated HeLa gDNA (Cat. #3520) is a HeLa-derived genomic DNA enzymatically methylated with CpG Methylase (M.Sss I) and purified by phenol/chloroform extraction and ethanol precipitation.

Episcope Unmethylated HCT116 DKO gDNA (Cat. #3521) is a genomic DNA purified from DNMT DKO HCT116 cells.

Episcope Methylated HCT116 gDNA (Cat. #3522) is a HCT116-derived genomic DNA enzymatically methylated with CpG Methylase (M.Sss I) and purified by phenol/chloroform extraction and ethanol precipitation.

Form

10 mM Tris-HCl (pH8.0)

1 mM EDTA

Storage

-20°C

Quality Control

Bisulfite sequencing was performed using EpiScope Methylated HeLa gDNA (Cat. #3520) to analyze the methylation levels of CpG islands on human MLH1 and GAPDH genes. It was confirmed with ≧95% accuracy that the CpG islands at those regions were methylated.

Using the Episcope Unmethylated HCT116 DKO gDNA (Cat. #3521), the methylation levels of 25 gene promoters were analyzed by methylated DNA enrichment using the EpiXplore™ Methylated DNA Enrichment Kit followed by EpiScope Promoter qPCR Array (Human) analysis with 2X SYBR® Premix EX Taq GC. It was confirmed that the methylation levels of all gene promoters were low.

Bisulfite sequencing was performed using EpiScope Methylated HCT116 gDNA (Cat. #3522) to analyze the methylation levels of CpG islands on human MLH1 and GAPDH genes. It was confirmed with ≧ 95% accuracy that the CpG islands at those regions were methylated.




 
 
Products
Cat. # Product Package Size Price # of Units Select
3520 EpiScope™ Methylated HeLa gDNA 15 ug $224.00
3521 EpiScope® Unmethylated HCT116 DKO gDNA 10 ug $153.00
3522 EpiScope® Methylated HCT116 gDNA 10 ug $153.00
 

 

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