Proline-rich AKT1 substrate 1 40 kDa (PRAS40) is a protein encoded by the AKT1S1 gene in humans. PRAS40 binds to 14-3-3 proteins when phosphorylated. PRAS40 is a subunit of the mammalian target of rapamycin complex 1 (mTORC1), and it negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. It inhibits RHEB-GTP-dependent mTORC1 activation. Although it is a substrate for AKT1 phosphorylation, PRAS40 can also be activated by AKT1-independent mechanisms. PRAS40 is also known as AKT1 substrate 1 (proline-rich), AKT1S1, proline-rich AKT1 substrate 1, lobe, and MGC2865.
These products are affinity-purified IgG antibodies that recognize human PRAS40 protein. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection or immunoprecipitation (IP) of human PRAS40 protein.