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Products >  Cell_Biology >  mTOR_Signaling >  Antibodies >  PRAS40_Human

Anti-Human PRAS40 Rabbit IgG Antibody

Proline-rich AKT1 substrate 1 40 kDa (PRAS40) is a protein encoded by the AKT1S1 gene in humans. PRAS40 binds to 14-3-3 proteins when phosphorylated. PRAS40 is a subunit of the mammalian target of rapamycin complex 1 (mTORC1), and it negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. It inhibits RHEB-GTP-dependent mTORC1 activation. Although it is a substrate for AKT1 phosphorylation, PRAS40 can also be activated by AKT1-independent mechanisms. PRAS40 is also known as AKT1 substrate 1 (proline-rich), AKT1S1, proline-rich AKT1 substrate 1, lobe, and MGC2865.

These products are affinity-purified IgG antibodies that recognize human PRAS40 protein. The antibodies were raised in rabbit using synthetic peptides and can be used for Western blot (WB) detection or immunoprecipitation (IP) of human PRAS40 protein.

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Features

  • Cat.#: 28033A, 28033B
  • Target Name: Human PRAS40 protein
  • Antigen: Human PRAS40 C-terminal synthetic peptide
  • Application: WB, IP
  • Target Entrez Gene ID: 84335

  • Cat.#: 28035A, 28035B
  • Target Name: Human PRAS40 protein (phosphorylated)
  • Antigen: Human PRAS40 (Ser-183 phosphorylated) C-terminal synthetic peptide
  • Application: WB
  • Target Entrez Gene ID: 84335

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 0.5–3.0 µg/mL (Cat.# 28033); 1–3 µg/mL (Cat.# 28035)
  • Immunoprecipitation: 1–3 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

AKT1 substrate 1 (proline-rich), AKT1S1, proline-rich AKT1 substrate 1, lobe, MGC2865




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
28033A Anti-Human PRAS40 (P238) Rabbit IgG Affinity Purify 10 ug $113.00 *
28033B Anti-Human PRAS40 (P238) Rabbit IgG Affinity Purify 100 ug $499.00
28035A Anti-Human PRAS40 (S183 Phosphorylated) Rabbit IgG Affinity Purify 10 ug $113.00 *
28035B Anti-Human PRAS40 (S183 Phosphorylated) Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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