Monoclonal Antibodies for detecting OX40 and OX40L
OX40 (CD134) and OX40L (CD252) are members of the tumor necrosis factor (TNF) superfamily of receptors and ligands, respectively. OX40 is expressed on activated lymphocytes, and signaling through OX40 generates strong costimulatory effects, including T cell proliferation, cytokine production, and T cell migration into tissues. These responses are mediated by OX40L expressed on antigen presenting cells, such as dendritic cells and activated B cells. Both OX40 and OX40L are constitutively expressed in human T cell leukemia virus type I (HTLV-I)-infected T cells. OX40 surface expression has been shown to be induced by a HTLV-I-encoded protein (Tax).
These monoclonal antibodies recognize either OX40 or OX40L. The antibodies were generated in mouse and can be used for detection of OX40 or OX40L by Western blot, immunoprecipitation, or flow cytometry.