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Products >  Cell_Biology >  Stem_Cell_Research >  Antibodies >  Sox2

Anti-Sox2 Monoclonal Antibody

SRY (sex determining region Y)-box 2 or Sox2 is a transcription factor required for the maintenance of undifferentiated embryonic stem cells. In addition to being an undifferentiated embryonic stem cell marker, Sox2 is one of four reported factors (OCT4, SOX2, NANOG, and LIN28) that are capable of reprogramming human somatic cells to an undifferentiated state. These induced pluripotent stem cells exhibit the essential characteristics of embryonic stem (ES) cells.

Anti-Sox2 monoclonal antibody is raised against the KLH-conjugated human Sox2 peptide sequence (219-236) [GSPTYSMSYSQQGTPGMA]. Human Sox2 (Sox 2) antibody may be used for Western blot and immunocytochemistry applications.

At-A-Glance Documents Images & Data

Features

  • Human Sox2 (Sox 2) antibody specifically reacts with human Sox2
  • Subclass: IgG2b
  • Clone: SOX 2 9B-12H
  • Form: Lyophilized

Applications

  • Western blot analysis under reducing conditions
  • Immunocytochemistry

 

Source :

Anti-Sox2 monoclonal antibody was obtained by fusing the mouse myeloma P3U1 cell line with C57BL/6 mouse lymph node cells following immunization with KLH-conjugated human Sox2 peptide sequence (219-236) [GSPTYSMSYSQQGTPGMA]. Human Sox2 (Sox 2) antibody was harvested from SCID mouse ascitic fluid.

Storage

4°C

This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at –20° for up to 1 year or at 4° for up to 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw. Diluted antibody should not be stored.

Notes

Store antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentrations may result in decreased product stability.

Reconstituted antibody solution should contain 0.1% sodium azide when stored at 4°C.

Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If further dilution is required, dilute with the Dilution Solution (listed below) just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution Solution.

Dilution Solution

  • 10 mM PBS (pH 7.4)
  • 1.0% BSA
  • (0.1% NaN3)*

* When stored at 4°C, 0.1% sodium azide should be added as a preservative.

References 

1. Rizzino, A. (2009) Wiley Interdiscip. Rev. Syst. Biol. Med. 1(2):228-236.
2.Chambers, I. & Tomlinson, S.R. (2009) Development 136(14):2311-2322. 
3. Yu, J. et al. (2007) Science 318(5858):1917-1920. 
4. Aasen, T. et al. (2008) Nature Biotechnology 26:1276–1284.
5. Takahashi, K. et al. (2007) Cell 131:861-872.




 
 
Products
Cat. # Product Package Size Price # of Units Select
M223 Sox2 (Human), Monoclonal 0.1 mg $306.00
 

 



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