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Products >  Cell_Biology >  Stem_Cell_Research >  Antibodies >  Oct4

Anti-Oct4 Monoclonal Antibody

Oct4 is an undifferentiated cell marker and is one of four reported factors (OCT4, SOX2, NANOG, and LIN28) capable of reprogramming human somatic cells to an undifferentiated state. These pluripotent stem cells also exhibit the essential characteristics of embryonic stem (ES) cells.

Anti-Oct4 monoclonal antibody is raised against recombinant full-length human Oct4 protein, an undifferentiated cell marker. Human Oct4 (Oct-4, Oct 4) antibody may be used for Western blot and immunocytochemistry applications.

At-A-Glance Documents Images & Data

Features

  • Human Oct4 (Oct-4, Oct 4) antibody specifically reacts with human Oct4
  • Subclass: IgG2b
  • Clone: OCT413-12F
  • Form: Lyophilized

Applications

  • Western Blot analysis under reducing conditions
  • Immunocytochemistry staining

 

Source

Anti-Oct4 monoclonal antibody was obtained by fusing the P3U1 mouse myeloma cell line with lymph node cells of C57BL/6 mouse after immunization with recombinant human Oct4 protein (NP_002692). The monoclonal antibody was harvested from ascitic fluid of SCID mouse.

Storage

4°C

This product does not contain preservative.

Notes

Store antibody at a minimum concentration of 2.0 mg/ml. Lower antibody concentrations may result in decreased product stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C. Avoid repeated freeze-thaw.

Notes:

Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration 2.0 mg/mL). This solution can be used as a stock solution. If further dilution is required, dilute with the Dilution Solution (listed below) just prior to use. When the entire amount of antibody is to be used over a short period of time, it may be dissolved directly in 500 μL or more of Dilution Solution.

Dilution Solution:

  • 10 mM PBS (pH 7.4)
  • 1.0% BSA
  • (0.1% NaN3)*

* When stored at 4°C, 0.1% sodium azide should be added as a preservative.

 

 

References

1. Nichols, J. et al. (1998) Cell 95:379-391.

2. Takahashi, K. et al. (2007) Cell 131:861-872.

3. Rizzino, A. et al. (2009) Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228-236.

4. Pesce, M. & Schöler, H.R. (2001) Stem Cells 19(4):271-278.



 
 
Products
Cat. # Product Package Size Price # of Units Select
M221 Oct4 (Human), Monoclonal 0.1 mg $306.00
 

 



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