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Products >  Cell_Biology >  Stem_Cell_Research >  Antibodies >  Neural_Progenitor_Cell_Antibodies

Antibodies to Neural Progenitor Cells: Bf1, Crx, Emx 1, Irx3, L7/Pcp2, Otp, Otx2, Rx, and Six3

Neural progenitor cells express characteristic protein biomarkers, and antibodies to these biomarkers allow analysis of cell differentiation during embryonic development and organogenesis. These antibodies are of particular use for studies in the field of regenerative medicine, may be used as neurogenesis differentiation markers with induced ES or iPS cells, and are suitable for immunohistochemistry on fixed tissue and western blotting. L7 antibodies allow detection of Purkinje cells, which are the output neurons of the cerebellar cortex. Otp antibodies can be used to identify differentiating hypothalamic neuroendocrine cells. Antibodies to Emx1 allow detection of this cerebral cortical marker during early embryogenesis. Six3 antibodies have been used to study generation of rostral hypothalamic-like progenitors from mouse ES cell-derived neuroectodermal cells. Otx2 antibodies have been used to study differentiation of retinal progenitors in embryonic neural retina and induced ES cells in culture. The transcription factor detected by anti-Brain Factor 1 (Bf1) antibodies localizes to cerebral nerve precursor cells in a rostral region called the telencephalon. Rx antibodies detect a transcription factor critical for protein expression in retinal progenitor cells. Antibodies to Iroquois Homeobox Protein 3 (Irx3) can be used to study early development of the central nervous system and the caudal nerves. Detection of the photoreceptor-specific transcription factor Cone-rod homeobox protein (Crx) with anti-Crx antibodies enables study of the development and differentiation of retinal photoreceptor cells and the pineal gland.

These polyclonal antibodies were developed in the laboratory of Yoshiki Sasai, M.D., PhD, Center for Developmental Biology (RIKEN).

  At-A-Glance   Documents   Images & Data

Neural Progenitor Cells: Antibody Specifications

Cat. #

Antibody to

Cell type detected

Antigen

Host species

Alternative names for target

Specificity

M194

Mouse L7/Pcp2

Purkinje progenitor cells

KLH-conjugated peptide [AALSF RRNSS PQPQT QAP] from the C-terminal region of mouse L7/Pcp2

Rabbit

Purkinje cell protein 2, Purkinje cell protein 2 homolog, Purkinje cell-specific protein L7

C-terminal region of mouse L7/Pcp2

M195

Mouse Otp

Hindbrain and hypothalamic neurons during embryonic development

KLH-conjugated peptide [LRRKA LEHTV SMSFT] from the C-terminal region of mouse Otp

Guinea pig

orthopedia homeobox, orthopedia homolog, homeobox protein orthopedia

C-terminal region of mouse Otp

M196

Mouse Emx 1

Cerebral cortex neurons during embryonic development

KLH-conjugated peptide [ESEQK KKGSH HINRW RIATK QANGE DIDVT SND] of mouse Emx1

Guinea pig

empty spiracles homeobox 1, Empty spiracles homolog 1, empty spiracles homolog 1 (Drosophila)

C-terminal region of mouse Emx1

M197

Mouse Six3

Rostral brain progenitors; forebrain and retina, early embryonic and CNS development

KLH-conjugated peptide [RLQHQ AIGPS GMRSL AEPGC] from the C-terminal region of mouse Six3

Rabbit

SIX homeobox 3, Sine oculis homeobox homolog 3, homeobox protein SIX3

C-terminal region of mouse Six3

M198

Mouse Otx2

Retinal photoreceptor cells (cone cells and rod cells) during embryonic development

KLH-conjugated peptide [CGSYL TPMHH QLPGP GATLS PMGTN] from the C-terminal region of mouse Otx2

Rabbit

Orthodenticle homolog 2, orthodenticle homolog 2 (Drosophila), homeobox protein OTX2

C-terminal region of mouse Otx2

M199

Human Otx2

Retinal photoreceptor cells (cone cells and rod cells) during embryonic development

KLH-conjugated peptide [CGSYL TPMHH QLPGP GATLS PMGTN] from the C-terminal region of human Otx2

Rabbit

As described above (M198)

C-terminal region of human Otx2

M227

Human-Mouse Bf1

Cerebral neural progenitor cells in the telencephalon

C-terminal peptide [SLPSF TTGLS GGLSD YFTHQ NQGSS SNPLI H] of human-mouse Bf1 conjugated with bovine thyroglobulin

Rabbit

Brain factor 1, Transcription Factor BF-1, forkhead box protein G1

C-terminal region of human and mouse Bf1

M228

Mouse Rx

Retinal progenitor cells

KLH-conjugated p eptide fragments of mouse or human/mouse Rx

Rabbit

Retinal homeobox protein

Mouse Rx

M229

Human-Mouse Rx

Retinal progenitor cells

As described above (M228)

Guinea pig

As described above (M228)

Human and Mouse Rx 

M230

Mouse Irx3

Neural plate progenitor cells involved in caudal nerve development 

KLH-conjugated peptide [CSALE VEKKL LKTAF QPVPR RPQNH LDAAL VLSAL SSS] from the C-terminal region of mouse Irx3

Rabbit

Iroquois related homeobox 3 protein

Mouse Irx3

M231

Human Crx

Retinal photoreceptor cells (cone cells and rod cells) during embryonic development

KLH-conjugated peptide fragments of human Crx

Rabbit

Cone-rod homeobox protein

Human Crx

Applications

  • Immunohistochemical staining on fixed tissue
  • Western blotting under reducing conditions (Cat. # M196, M197, M228, M229, M230, M231)

Form

Frozen solution in 10 mM PBS, pH 7.4. If dilution is necessary for your experimental application, dilute in a solution of 10 mM PBS pH 7.4 and 1.0% BSA just prior to use. Do not store diluted antibody.

Concentration

0.2 mg IgG per 100 µl

Storage

Store the stock solution (2.0 mg/ml) in aliquots at -20°C. Stock solution may be stored at 4°C for up to 6 months after adding sodium azide to a final concentration of 0.1%. Diluted antibody is prone to degradation; do not store at a concentration of less than 2.0 mg/ml.




 
 
Products
New Offers Cat. # Product Contents Size Price Units Select
  M194 Polyclonal Antibody to Mouse L7/Pcp2 100 uL $386.00
  M195 Polyclonal Antibody to Mouse Otp 100 uL $386.00
  M196 Polyclonal Antibody to Mouse Emx1 100 uL $386.00
  M197 Polyclonal Antibody to Mouse Six3 100 uL $386.00
  M198 Polyclonal Antibody to Mouse Otx2 100 uL $386.00
  M199 Polyclonal Antibody to Human Otx2 100 uL $386.00
M227 Anti-Human/Mouse Bf1, Polyclonal 0.2 mg $386.00
M228 Anti-Mouse Rx, Polyclonal 0.2 mg $386.00
M229 Anti-Human/Mouse Rx, Polyclonal (Guinea Pig) 0.2 mg $386.00
M230 Anti-Mouse Irx3, Polyclonal 0.2 mg $386.00
M231 Anti-Human Crx, Polyclonal 0.2 mg $386.00
 

 

 
 

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