Adipocyte differentiation is an important focus of scientific research because adipose tissue plays an essential role in energy homeostasis. Adipocyte differentiation as a function of transcriptional and epigenetic regulation is also a popular field of investigation. Mouse embryonic fibroblast 3T3-L1 cells (ATCC No. CL-173) are one of the most commonly used cell models in the study of adipocyte differentiation because they easily differentiate into adipocytes when induced by insulin, 3-isobutyl-1-methylxanthine and dexamethasone.
The Adipoinducer Reagent for Mammalian Cells efficiently induces adipocyte differentiation in mouse embryonic fibroblast 3T3-L1 cells, rat brown/white preadipocytes, mouse/rat/rabbit bone marrow cells and other animal cells. This kit contains three of the most frequently used inducers of adipocyte differentiation: insulin, 3-isobutyl-1-methylxanthine and dexamethasone. Adipocyte differentiation can be achieved by culturing cells in an appropriate medium containing the aforementioned inducer agents.