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Products >  Cell_Biology >  Signal_Transduction >  Reagents >  Adipoinducer_Reagent-for_animal_cell

Adipoinducer Reagent for Mammalian Cells

Adipocyte differentiation is an important focus of scientific research because adipose tissue plays an essential role in energy homeostasis. Adipocyte differentiation as a function of transcriptional and epigenetic regulation is also a popular field of investigation. Mouse embryonic fibroblast 3T3-L1 cells (ATCC No. CL-173) are one of the most commonly used cell models in the study of adipocyte differentiation because they easily differentiate into adipocytes when induced by insulin, 3-isobutyl-1-methylxanthine and dexamethasone.

The Adipoinducer Reagent for Mammalian Cells efficiently induces adipocyte differentiation in mouse embryonic fibroblast 3T3-L1 cells, rat brown/white preadipocytes, mouse/rat/rabbit bone marrow cells and other animal cells. This kit contains three of the most frequently used inducers of adipocyte differentiation: insulin, 3-isobutyl-1-methylxanthine and dexamethasone. Adipocyte differentiation can be achieved by culturing cells in an appropriate medium containing the aforementioned inducer agents.

 

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Features

  • Contains three commonly used adipocyte differentiation-inducers: insulin, 3-isobutyl-1-methylxanthine and dexamethasone
  • Efficiently induces adipocyte differentiation in mouse embryonic fibroblast 3T3-L1 cells, rat brown/white preadipocytes, mouse/rat/rabbit bone marrow cells and other animal cells

Kit Components

Kit contains 3 sets of reagents (for 100 mL differentiation medium + 200 mL maintenance medium)

Additive reagents for differentiation medium
Insulin solution (final conc. 10 µg/mL)*1 mL x 3
Dexamethasone solution (final conc. 2.5 µM)*0.5 mL
3-isobutyl-1-methylxanthine solution (final conc. 0.5 mM)*0.1 mL
Additive reagent for maintenance medium
Insulin solution (final conc. 10 µg/mL)*2 x 1 mL

* Final concentration after the specified volume is added to the medium

Storage

–20°C

References

1. Choi, K.M. et al. (2011) Biol. Pharm. Bull. 34(6):912-916.

2. Janderová, L. et al. (2003) Obes. Res. 11(1):65-74.


 
Products
Cat. # Product Contents Size Price Units Select
MK429 AdipoInducer Reagent (for animal cell) 3 Rxns $159.00 *
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