This reagent efficiently induces adipocyte differentiation in mouse embryonic fibroblast 3T3-L1 cells (ATCC No. CL-173), rat brown/white preadipocytes, mouse/rat/rabbit bone marrow cells and other animal cells. This kit contains three of the most frequently used adipocyte differentiation-inducers: insulin, 3-isobutyl-1-methylxanthine and dexamethasone. Adipose differentiation can be achieved by culturing cells in an appropriate medium containing these inducer reagents.
Adipocyte differentiation is an area of scientific focus because adipose tissue plays an important role in energy homeostasis. Researchers are studying transcriptional regulation of adipocyte differentiation, and the role of epigenetic regulation. 3T3-L1 cells are one of the models used to study this process because they differentiate efficiently into adipocytes when induced by insulin, 3-isobutyl-1-methylxanthine and dexamethasone.
