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Takara's EIA Kit

The Takara EIA Kit is a complete system for measuring specific molecules present in biological samples such as plasma, serum, urine and cultured cell extracts. The majority of these kits are based on a sandwich-type enzyme immunoassay (EIA), which utilizes a pair of monoclonal antibodies, each recognizing a different epitope on the antigen molecule. Quantitative measurement of antigen binding is mediated by enzymatic color formation in 96-well microtiter plates.

Principle

One of the antibodies in the pair is supplied pre-labeled with peroxidase (POD), and the other clone is precoated onto the microtiter plate. Specimens (or standard solutions) are incubated in the coated microtiter plate wells, and the antigen is captured onto the plate. After washing the plate, the labeled antibody is added to the wells and another incubation step is performed, tagging the bound antigen with POD-labeled antibody.

Reaction between the plate-bound POD and chromogenic substrate (H2O2, and TMBZ) generates production of colored reaction product. The quantity of reaction product can be measured by a plate reader and is proportional to the amount of antigen present in the specimens or standards.

Assay Duration

The incubation and detection steps on pre-coated microtiter plates require approximately 3 hours.

Total Assay Capacity

96 assays

Assay Capacity for Test Sample

If all assays (including standards) are run in duplicate, 48 reactions are possible.

Test Sample Type

These assays can generally be conducted on human serum, plasma, or urine; culture supernatants; or cell extracts. Recommended sample types may vary by kit.

Specimen Volume Required

If each test sample is run in duplicate, approximately 210 µL (i.e., 100 µL per assay plus 10 µL for each sample transfer) is required. One-step sandwich EIAs require 50 µL.

Standard Curves

Since conditions may vary from assay to assay, a standard curve must be established for every run. Refer to user manual for more information if culture supernatants are to be measured.

Storage

Store at 2 to 8°C

Summary of Takara EIA Kits

Product Cat.# Assay Range Sensitivity Principle Package
Size
E-cadherin MK117 84.4–2,700 ng/ml 84.4 ng/ml Precoated Two-step sandwich 96 assays
PIP (Procollagen type I C-Peptide) MK101 10–640 ng/ml 10 ng/ml Precoated One-step sandwich 96 assays
RetroNectin EIA Kit MK140 3.1–200 ng/ml 3.1 ng/ml Precoated Two-step sandwich 96 assays
Fibronectin MK115 25–800 ng/ml 25 ng/ml Precoated Two-step sandwich 96 assays
Vitronectin MK102 5–320 ng/ml 5 ng/ml Precoated One-step sandwich 96 assays
Laminin MK107 5–320 ng/ml 5 ng/ml Precoated Two-step sandwich 96 assays
Gla-Osteocalcin MK111 0.5–16 ng/ml 0.5 ng/ml Precoated Two-step sandwich 96 assays

Undercarboxylated-Osteocalcin

MK118 0.25–8 ng/ml 0.25 ng/ml Precoated Two-step sandwich 96 assays
Rat Gla-OC Competitive MK126 0.25–16 ng/ml 30 ng/ml Precoated Two-step sandwich 96 assays
Rat Glu-OC Competitive MK146 0.125–8 ng/ml 10 ng/ml Precoated Two-step sandwich 96 assays
Rat Gla/Glu-Osteocalcin MK147 Gla-OC: 0.25–16 ng/ml;
Glu-OC: 0.125–8 ng/ml
0.25 ng/ml; 0.125 ng/ml Precoated Two-step sandwich 1 set
Human Gla-Osteocalcin MK128 0.2–12 ng/ml 0.2 ng/ml Precoated Two-step sandwich

96 assays

Mouse Gla-OC High Sensitive EIA KIT MK127 0.5–16 ng/ml 0.5 ng/ml Precoated Two-step sandwich 96 assays
Pig Gla-Osteocalcin EIA Kit MK139 1.0–64.0 ng/ml 1.0 ng/ml Precoated Two-step sandwich 96 assays
Pig Glu-Osteocalcin EIA Kit MK149 0.5–32.0 ng/ml 0.5 ng/ml Precoated Two-step sandwich 96 assays
Influenza Virus Typing Set MK421 Equivalent to PAP method Avidin-biotin method 96 assays
Rat Heme Oxygenase-1 MK124 0.125–8.0 ng/ml 0.125 ng/ml Precoated Two-step sandwich 96 assays
Mouse Heme Oxygenase-1 MK125 0.125–8.0 ng/ml 0.125 ng/ml Precoated Two-step sandwich 96 assays
Glycocalicin MK105 10–640 ng/ml 10 ng/ml Precoated Two-step sandwich 96 assays

The EIA Kits are for research use only. They should not be used in diagnostic or therapeutic procedures.

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