Applications

• Detection of human Sox2 by Western Blot analysis under reducing condition
• Immunocytochemistry staining

Source :

Monoclonal antibody was obtained by fusing the P3U1 mouse myeloma cell line with lymph node cells of C57BL/6 mouse after immunization with KLH-conjugated peptide from human Sox2 sequence (amino acid 219-236 : GSPTYSMSYSQQGTPGMA). The monoclonal antibody was harvested from ascitic fluid of SCID mouse.

Storage

4°C

This product does not contain preservatives. The stock solution (2.0 mg/mL) should be stored in aliquots at –20° for 1 year, or should be stored at 4° for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.

Notes

Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability.

Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution solution.

Dilution Solution

• 10 mM PBS (pH7.4)
• 1.0％ BSA
• (0.1％ NaN₃)*

* When stored at 4°C, 0.1% sodium azide should be added as a preservative.