Applications

• Western Blot analysis under reducing conditions
• Immunocytochemistry staining

Source

Anti-Oct4 monoclonal antibody was obtained by fusing the P3U1 mouse myeloma cell line with lymph node cells of C57BL/6 mouse after immunization with recombinant human Oct4 protein (NP_002692). The monoclonal antibody was harvested from ascitic fluid of SCID mouse.

Storage

4°C

This product does not contain preservative.

Notes

Store antibody at a minimum concentration of 2.0 mg/ml. Lower antibody concentrations may result in decreased product stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C. Avoid repeated freeze-thaw.

Notes:

Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration 2.0 mg/mL). This solution can be used as a stock solution. If further dilution is required, dilute with the Dilution Solution (listed below) just prior to use. When the entire amount of antibody is to be used over a short period of time, it may be dissolved directly in 500 μL or more of Dilution Solution.

Dilution Solution:

• 10 mM PBS (pH 7.4)
• 1.0％ BSA
• (0.1％ NaN₃)*

* When stored at 4°C, 0.1% sodium azide should be added as a preservative.

References

1. Nichols, J. et al. (1998) Cell 95:379-391.

2. Takahashi, K. et al. (2007) Cell 131:861-872.

3. Rizzino, A. et al. (2009) Wiley Interdiscip. Rev. Syst. Biol. Med. 1:228-236.