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Products >  Cell_Biology >  Miscellaneous >  Secondary_Antibodies >  Monoclonal_Secondary_Antibody

Unconjugated Secondary Antibodies

Takara offers a series of unconjugated secondary antibodies derived from IgG (with one antibody derived from IgY) that can be conjugated to any label of choice (e.g., horseradish peroxidase or alkaline phosphatase). Once labeled, any listed secondary antibody may be used for Western blot, immunoprecipitation, staining and antibody capture EIA.

At-A-Glance Documents Images & Data

Features

  • Minimization of lot-to-lot variability due to monoclonal source 
  • Conjugation of secondary antibody to label of choice

Applications

  • Western blot*
  • Immunoprecipitation*

*See datasheet for individual antibody-specific applications

Source

Product codeClone No.
M281MS-G 20-10DMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with SD (Sprague-Dawley) rat lymph cells following immunization with mouse serum IgG. The monoclonal antibody was harvested from SCID mouse ascitic fluid.
M282RAT-G 31A-12HMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with C57BL/6 mouse lymph cells following immunization with rat serum IgG. The monoclonal antibody was harvested from SCID mouse ascitic fluid.
M283RAB-G 26-1Monoclonal antibody was obtained by fusing the P3U1 myeloma cell line with C57BL/6 mouse lymph cells following immunization with rabbit serum IgG. The monoclonal antibody was harvested from SCID mouse ascitic fluid.
M284SHP-G 5-10EMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with BALB/c mouse lymph cells following immunization with sheep serum IgG. The monoclonal antibody was harvested from BALB/c mouse ascitic fluid.
M285SHP-G 21-8AMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with BALB/c mouse lymph cells following immunization with sheep serum IgG. The monoclonal antibody was harvested from BALB/c mouse ascitic fluid.
M286GP-G 10A-12EMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with C57BL/6 mouse lymph cells following immunization with Guinea pig serum IgG. The monoclonal antibody was harvested from SCID mouse ascitic fluid.
M287HU-G 13-9HMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with BALB/c mouse lymph cells following immunization with human serum IgG. The monoclonal antibody was harvested from BALB/c mouse ascitic fluid.
M288CHK-Y 25-6AMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with C57BL/6 mouse lymph cells following immunization with chicken serum IgY. The monoclonal antibody was harvested from SCID mouse ascitic fluid.

Specificity

Product codeClone No.SpecificitySubclass
M281MS-G 20-10D
  • Reacts specifically with mouse IgG Fab.
  • Does not react with mouse IgM.
  • Does not react with rat, rabbit, guinea pig, goat, sheep and human IgG or chicken IgY.
Rat IgG1
M282RAT-G 31A-12H
  • Reacts specifically with rat IgG Fab.
  • Does not react with mouse, rabbit, guinea pig, sheep, goat and human IgG or chicken IgY.
Mouse IgG1
M283RAB-G 26-1
  • Reacts specifically with rabbit IgG Fab.
  • Does not react with mouse, rat, guinea pig, sheep, goat and human IgG or chicken IgY.
Mouse IgG1
M284SHP-G 5-10E
  • Reacts specifically with sheep IgG Fab.
  • Does not react with mouse, rat, rabbit, guinea pig, goat, and human IgG or chicken IgY.
Mouse IgG1
M285SHP-G 21-8A
  • Reacts specifically with sheep and goat IgG.
  • Does not react with mouse, rat, rabbit, guinea pig and human IgG or chicken IgY.
Mouse IgG 2b
M286GP-G 10A-12E
  • Reacts specifically with guinea pig IgG.
  • Does not react with mouse, rat, rabbit, human, sheep and goat IgG or chicken IgY and IgG.
Mouse IgG1
M287HU-G 13-9H
  • Reacts specifically with human IgG Fc.
  • Does not react with mouse, rat, rabbit, guinea pig, sheep and goat IgG or chicken IgY and IgG.
Mouse IgG1
M288CHK-Y 25-6A
  • Reacts specifically with chicken IgY Fab.
  • Does not react with mouse, rat, rabbit, guinea pig, sheep, goat and human IgG.
Mouse IgG1

Storage

-20 ℃

This product does not contain preservative.

The stock solution (2.0 mg/ml) should be stored in aliquots at -20℃ for up to 1 year or at 4℃ for up to 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw. Diluted antibody should not be stored.

Form

Frozen solution (10 mM PBS, pH 7.4)




 
 
Products
Cat. # Product Package Size Price # of Units Select
M281 Anti-Mouse IgG, Monoclonal (MS-G 20-10D) 0.2 mg $153.00
M282 Anti-Rat IgG, Monoclonal (RAT-G 31A-12H) 0.2 mg $153.00
M283 Anti-Rabbit IgG, Monoclonal (RAB-G 26-1) 0.2 mg $153.00
M284 Anti-Sheep IgG, Monoclonal (SHP-G 5-10E) 0.2 mg $153.00
M285 Anti-Goat/Sheep IgG, Monoclonal (SHP-G 21-8A) 0.2 mg $153.00
M286 Anti-Guinea Pig IgG, Monoclonal (GP-G 10A-12E) 0.2 mg $153.00
M287 Anti-Human IgG, Monoclonal (HU-G 13-9H) 0.2 mg $153.00
M288 Anti-Chicken IgY, Monoclonal (CHK-Y 25-6A) 0.2 mg $255.00
 

 


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