The Rat IgG EIA Kit is a quantitative enzyme-linked immunosorbant assay (ELISA) that uses rat-specific polyclonal antibody to IgG. This kit can be used not only to measure IgG in rat blood samples regardless of strain, but it can also monitor antibody production in hybridoma cells. This kit is supplied with a ready-to-use plate coated with a solid-phase capture antibody. Kit usage and dosage instructions are designed to allow for simple yet highly sensitive detection of rat IgG in only 60 minutes.
Immunoglobulins (Ig) are proteins that play a key role in antibody-mediated immunity, one of the host's key defense mechanisms. There are 5 classes of immunoglobulins (IgG, IgA, IgM, IgE and IgD) present in animals, with IgG accounting for 80% of immunoglobulins found in blood. IgG levels vary greatly depending on immune response; however, IgG is generally present in the blood from 3–10 mg/mL. This is a high enough concentration for IgG to be easily detected without the need for a high sensitivity ELISA.
Monoclonal antibodies are single clone immunoglobulins produced by hybridoma cells, which are a fusion between antibody-forming cells and tumor cells grown in culture. Monoclonal antibody production technology is now well established, with it being relatively easy to construct heterohybridomas through the fusion of rat lymphoma cells with mouse myeloma cells. However, unlike the IgG antibody levels measured in blood, heterohybridoma supernatant antibody levels are low and require a high sensitivity assay for detection. The Rat IgG EIA Kit is therefore indispensible for assaying rat IgG production during the maintenance of rat monoclonal antibody-producing heterohybridomas.