The Mouse IgG EIA Kit is a quantitative enzyme-linked immunosorbant assay (ELISA) that uses mouse-specific polyclonal antibody to IgG. This kit can be used to measure IgG levels in mouse blood, test the quality of monoclonal antibody-based drugs or research reagents, and monitor antibody production in hybridoma cells. This kit is supplied with a ready-to-use plate coated with a solid-phase capture antibody. Kit usage and dosage instructions are designed for sensitive and reproducible detection of mouse immunoglobulin. For high sensitivity detection, the mouse immunoglobulin G (IgG) ELISA requires only 60 minutes from start to completion.
Immunoglobulins are proteins that play a key role in antibody-mediated immunity. There are five classes of immunoglobulins (IgG, IgA, IgM, IgE and IgD) present in animals, with IgG accounting for 80% of all immunoglobulins circulating in blood. IgG levels vary greatly depending on immune response; however, IgG is generally present at 3–10 mg/mL in blood. Such a high concentration of IgG indicates that this antibody can be detected without the need for a highly sensitive IgG ELISA.
Monoclonal antibodies are uniform immunoglobulins produced by a single colony of hybridoma cells that respond to the same epitope. Because of these characteristics, monoclonal antibodies are widely used as research reagents and as the main component of some disease therapeutics. Hybridomas are a fusion of antibody-forming cells and tumor cells grown in culture. Scientists working with hybridoma cells monitor immunoglobulin production by detecting mouse IgG in the hybridoma culture supernatant. Unlike blood IgG levels, however, hybridoma supernatant IgG levels are typically low. Thus, a highly sensitive EIA kit is required for detection.