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Products >  Cell_Biology >  Miscellaneous >  Reagents_Kits >  IgG-Human_EIA_Kit

Human Immunoglobin G (IgG) ELISA: Human IgG EIA Kit

The Human IgG EIA Kit is a colorimetric enzyme-linked immunosorbant assay (ELISA) for quantitative determination of human IgG in human blood and body fluid samples. IgG is produced by activated B lymphocytes (plasma cells) and functions in many roles including immunity, infection and opsonization.

IgG is a plasma cell protein that is produced within the lymph nodes, spleen, bone marrow, thymus, small intestinal mucosa, respiratory tract mucosa and other tissues. It is the only immunoglobulin that can cross the placenta. Since IgG is produced as part of the body's response to bacteria, viruses, drugs and tissue antigens, measurement of blood IgG levels can reveal hyper-responsiveness to antigen stimulation or physiological anomalies at IgG-producing sites, among other disorders. Thus, human IgG levels are often measured during allergy and immune response testing. The presence of high IgG levels has been linked to polyclonal hypergammaglobulinemia, collagen disease, asymptomatic M-proteinemia, chronic infection, myeloma, IgG multiple myeloma and other pathological conditions. Low IgG levels, on the other hand, have been correlated with disorders such as agammaglobulinemia, hypogammaglobulinemia, severe immunodeficiency and nephrosis syndrome.

Note: this product is for research use only.

At-A-Glance Documents Images & Data

Features

  • Sensitive and quantitative measurement of human IgG.
  • High sensitivity sandwich ELISA that comes with a ready-to-use plate coated with solid-phase capture antibody

Applications

  • Quantitative determination of human IgG in human blood and body fluid samples
  • Allergy and immune response testing
  • ELISA

Components

Antibody Coated Microtiter plate1 plate (8 wells × 12 strips)
Antibody - Peroxidase Conjugate1 vial (for 11 mL)
Standard1 vial (for 1 mL)
Sample Diluent2 vials (11 mL × 2)
Substrate Solution1 vial (12 mL)

Storage

4ºC

Performance

  • Range of assay: 10–640 ng/mL
  • Sensitivity: 10.0 ng/mL
  • Assay duration: 2.5 hours
  • Specificity: Human IgG
  • Test specimen type: Human blood and bodily fluids. When using human blood, the recommended dilution is >105 fold.
  • Specimen volume required: 100 µL/assay well



 
 
Products
Cat. # Product Package Size Price # of Units Select
MK136 Human IgG EIA Kit 96 Rxns $653.00
 

 


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