The enzyme glycerol 3-phosphate dehydrogenase (GPDH) catalyzes a reversible reaction between dihydroxyacetone phosphate (DHAP) and glycerol 3-phosphate. NAD is utilized as a coenzyme. GPDH activity increases significantly during adipocyte differentiation; as a result, GPDH activity measurement is used to monitor fat synthesis.
The Glycerol 3-Phosphate Dehydrogenase or GPDH Activity Assay Kit is a 96-well format, microtiter plate assay designed to measure the activity of GPDH. This kit can be used to study mechanisms that regulate adipocyte differentiation wherein primary or established progenitor cells differentiate into adipocytes. The assay procedure involves cell extract generation via the addition of the Enzyme Extraction Buffer followed by serial dilution of the extract. The serially diluted extract is then added to a 96-well plate containing GDPH Substrate solution and GPDH activity is calculated by measuring the change (decrease) in OD340 absorbance using a microtiter plate reader.