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Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_O-S >  Protein_S_Monoclonal

Anti-Protein S Monoclonal Antibody

Anti-Protein S Monoclonal Antibody can be used for detection of ProS2-fused proteins expressed from the cold shock expression vector pCold ProS2 DNA (Cat. #3371). ProS2 is an approximately 23 kDa soluble tandem-dimer peptide tag derived from the N-terminal domain of Protein S, a soluble protein that originates from the myxobacterium species Myxococcus xanthus.

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Applications

  • Protein S expression from pCold ProS2 DNA
  • Detection of ProS2-fused protein
  • Western blot analysis under reducing and non-reducing conditions (0.5–1 µg/mL)
  • Immunoprecipitation (10 µg/mL)
  • Use with cold shock expression vector pCold ProS2 DNA

Source

  • Clone: ProS 7B-8F
  • Immunogen: ProS2* tag protein
  • Immunized host: C57BL/6 mouse
  • Subclass: IgG1
  • Antibody titer: 1:2000

* ProS2 is approximately 23 kDa and is a tandem-dimer peptide derived from the N-terminal domain of the soluble myxobacterium Myxococcus xanthus Protein S. It is also expressed in the cold shock expression vector pCold ProS2 DNA (Cat. #3371).

Storage

Store at 4°C. The stock (2.0 mg/mL) and Reconstitution Solution should be stored in aliquots at –20°C for up to 1 year or at 4°C for up to 6 months. Avoid repeated freeze-thaw. The diluted antibody should not be stored.

Properties

  • Form: Lyophilized
  • Reconstitution Solution: 0.1% NaN3 (in water)
  • Reconstitution: Dissolve the lyophilized antibody in 50 µL of Reconstitution Solution (final concentration: 2.0 mg/mL).
  • This product does not cross-react with proteins derived from E. coli or the TF (Trigger Factor) tag.

References

1. Kobayashi, H. et al. (2009) Appl. Environ. Microbiol. 75:5356-5362.



 
 
Products
Cat. # Product Contents Size Price Units Select
M200 Monoclonal Anti-Pro S2, (Clone ProS 7B-8F) 0.1 mg $427.00
 

 

 
 

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