Takara
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_O-S >  Podocin_Human

Anti-Human Podocin Rabbit IgG Antibody

Podocin is a component of the slit diaphragm of podocytes, cells that are located in the Bowman's capsule in the kidneys. In humans, it is encoded by the NPHS2 gene. In the glomerulus, blood is filtered through the slit diaphragms before passing into nephrons. Podocin is one of several proteins that control glomerular permeability. It acts as a linker between the plasma membrane and the cytoskeleton. Mutations in the NPHS2 gene cause autosomal recessive steroid-resistant nephrotic syndrome (SRNS), a disease that rapidly progresses to end-stage renal disease (ESRD). Podocin has also been implicated in the development of other nephropathies, such as focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD). Podocin is also known as nephrosis 2, idiopathic, steroid-resistant (podocin); PDCN, and SRN1.

This product is an affinity-purified IgG antibody that recognizes human podocin protein. The antibody was raised in rabbit using a synthetic peptide and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or immunoprecipitation (IP) of human podocin protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 29040A, 29040B
  • Target Name: Human podocin protein
  • Antigen: Human podocin synthetic peptide
  • Application: WB, IHC, IP
  • Specificity: Cross-reacts with rat and mouse podocin
  • Target Entrez Gene ID: 7827

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 0.1 µg/mL
  • Immunohistochemical techniques: 1–2 µg/mL
  • Immunoprecipitation: 1–2 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

Nephrosis 2, idiopathic, steroid-resistant (podocin); NPHS2, PDCN, SRN1




 
 
Products
Cat. # Product Package Size Price # of Units Select
29040A Anti-Human Podocin Rabbit IgG Affinity Purify 10 ug $113.00 *
29040B Anti-Human Podocin Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions