Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_O-S >  PDGFR_Beta_Human_Mono

Anti-Human PDGFR-beta (22B1) Mouse IgG MoAb

Platelet-derived growth factor receptors (PDGFRs) are cell-surface tyrosine kinase receptors for members of the platelet-derived growth factor (PDGF) family. Two forms of PDGFR are known: PDFGR-alpha, encoded by the PDGFRA gene, and PDGFR-beta, encoded by the PDGFRB gene. The type of ligand bound to PDGFR determines whether the functional receptor consists of a homodimer or heterodimer of alpha and beta polypeptides. Binding of PDGF isoforms to PDGFR-beta initiates intracellular signaling through the MAPK, PI3K, and PKC-gamma pathways. A chromosomal translocation that that fuses the PDGFRB gene to the ETV6 leukemia gene results in a chronic myeloproliferative disorder with eosinophilia. PDGFR-beta is also known as platelet-derived growth factor receptor, beta polypeptide; platelet-derived growth factor receptor 1 (PDGFR1), CD140 antigen-like family member B (CD140B), and JTK12.

This product is an affinity-purified IgG antibody that recognizes human PDGFR-beta protein. The antibody was raised in mouse using recombinant PDGFR-beta protein and can be used for Western blot (WB) detection, immunoprecipitation (IP), neutralization assay (NT), or flow cytometry (FCM) of human PDGFR-beta protein.

  At-A-Glance   Documents   Images & Data

Features

  • Cat.#: 10351A, 10351B
  • Target Name: Human PDGFR-beta protein
  • Antigen: Human PDGFR-beta (extracellular domain) recombinant protein
  • Application: WB, IP, FCM
  • Target Entrez Gene ID: 5159

  • Cat.#: 10351F
  • Target Name: Human PDGFR-beta protein
  • Antigen: Human PDGFR-beta (extracellular domain) recombinant protein
  • Application: WB, IP, NT, FCM
  • Target Entrez Gene ID: 5159

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–5 µg/mL
  • Immunoprecipitation: 3–5 µg per reaction
  • Neutralization assay: 10 µg/mL
  • Flow cytometry: 0.1–0.5 µg (per 1 x 105 cells)

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.*
*Note: Cat.# 10351F is supplied frozen without BSA or NaN3.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).*

Alternative names

Platelet-derived growth factor receptor, beta polypeptide; PDGFRB, platelet-derived growth factor receptor 1, PDGFR1, CD140 antigen-like family member B, CD140B, JTK12




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
10351A Anti-Human PDGFR beta (22B1) Mouse IgG MoAb 10 ug $113.00 *
  10351B Anti-Human PDGFR beta (22B1) Mouse IgG MoAb 100 ug $499.00
  10351F Anti-Human PDGFR beta (22B1) Mouse IgG MoAb (Frozen) 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions