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Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_H-N >  Nucleic_Acid_Metabolism_Enzymes

Nucleic Acid Metabolism Enzyme Antibody

A variety of enzymes catalyze reactions involved in the synthesis, modification, and degradation of nucleic acids within cells. Many of these enzymes are being targeted in the development of anticancer drugs.

Thymidylate synthetase (TS) is used to synthesize thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. It is a target of anticancer drugs such as 5-fluorouracil and folate analogs. TS is also known as TMS, TSase, and HsT422.

Dihydropyrimidine dehydrogenase (DPD) is one of the enzymes required for pyrimidine degradation, the initial and rate-limiting step in pyrimidine catabolism. DPD catalyzes the reduction of uracil and thymine. It is a traget of the anticancer drug 5-fluorouracil. DPD is also known as dihydrothymine dehydrogenase, dihydrouracil dehydrogenase, DHP, dihydropyrimidine dehydrogenase [NADP(+)], and DHPDHase.

Orotate phosphoribosyltransferase (OPRT) catalyzes the formation of orotidine 5'-monophosphate (OMP) from orotate and phosphoribosyl pyrophosphate, as part of the pyrimidine biosynthetic pathway. In yeast and bacteria, OPRT is an independent enzyme with a unique gene coding for the protein; in higher eukaryotes, the catalytic function is performed by a subdomain of the enzyme uridine monophosphate (UMP) synthase. Human OPRT is also known as uridine monophosphate synthetase, UMP synthase, OMPdecase, OPRTase, orotidine 5'-phosphate decarboxylase, and uridine 5'-monophosphate synthase.

These products are affinity-purified IgG antibodies that recognize TS, DPD, and OPRT. The antibodies were raised in rabbit or mouse using recombinant protein or a synthetic peptide and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of TS, DPD, and OPRT.

At-A-Glance Documents

Features

  • Cat.#: 10409A, 10409B
  • Target Name: Human TS protein
  • Antigen: Human TS protein
  • Application: WB, IHC
  • Target Entrez Gene ID: 7298

  • Cat.#: 10411A, 10411B
  • Target Name: Human DPD protein
  • Antigen: Human DPD protein
  • Application: WB, IHC
  • Target Entrez Gene ID: 1806

  • Cat.#: 28135A, 28135B
  • Target Name: Human OPRT protein
  • Antigen: Human OPRT synthetic peptide
  • Application: WB, IHC
  • Target Entrez Gene ID: 7372

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1 µg/mL
  • Immunohistochemical techniques: 0.5 µg/mL (Cat.# 10409); 2 µg/mL (Cat.# 10411); 1 µg/mL (Cat.# 28135)

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

Thymidylate synthetase: TS, TMS, TSase, HsT422

Dihydropyrimidine dehydrogenase: DPD, dihydrothymine dehydrogenase, dihydrouracil dehydrogenase, DHP, dihydropyrimidine dehydrogenase [NADP(+)], DHPDHase

Orotate phosphoribosyltransferase: OPRT, uridine monophosphate synthetase, UMP synthase, OMPdecase, OPRTase, orotidine 5'-phosphate decarboxylase, uridine 5'-monophosphate synthase




 
 
Products
Cat. # Product Package Size Price # of Units Select
10409A Anti-TS (ATYMSMAB) Mouse IgG MoAb 10 ug $114.00 *
10409B Anti-TS (ATYMSMAB) Mouse IgG MoAb 100 ug $525.00
10411A Anti-DPD (ADPYDMAB) Mouse IgG MoAb 10 ug $114.00 *
10411B Anti-DPD (ADPYDMAB) Mouse IgG MoAb 100 ug $525.00
28135A Anti-OPRT Rabbit IgG Affinity Purify 10 ug $114.00 *
28135B Anti-OPRT Rabbit IgG Affinity Purify 100 ug $525.00
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