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Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_H-N >  Nephrin_Human

Anti-Human Nephrin Rabbit IgG Antibody

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that is required for the proper functioning of the glomerular filtration barrier in the kidney. The glomerular filtration barrier consists of fenestrated endothelial cells, the glomerular basement membrane, and specialized epithelial cells known as podocytes. Nephrin is found at the slit diaphragm of glomerular podocytes. The slit diaphragm functions as an ultrafilter to exclude albumin and other plasma macromolecules during the formation of urine. Mutations in the nephrin gene result in a disease known as Finnish-type congenital nephrosis 1, characterized by severe proteinuria (leakage of large amounts of protein into the urine). Nephrin is also known as nephrosis 1, congenital, Finnish type; CNF, NPHN, NPHS1, and renal glomerulus-specific cell adhesion receptor.

These products are affinity-purified IgG antibodies that recognizes human nephrin protein. The antibody was raised in rabbit using recombinant protein or a synthetic peptide, and can be used for Western blot (WB) detection, immunohistochemical (IHC) detection, or immunoprecipitation (IP) of human nephrin protein.

At-A-Glance Documents Images & Data

Features

  • Cat.#: 29050A, 29050B
  • Target Name: Human nephrin protein
  • Antigen: Recombinant human nephrin protein
  • Application: IHC
  • Target Entrez Gene ID: 4868

  • Cat.#: 29070A, 29070B
  • Target Name: Human nephrin protein
  • Antigen: Human nephrin C-terminal synthetic peptide
  • Application: WP, IHC, IP
  • Specificity: Cross-reacts with rat nephrin
  • Target Entrez Gene ID: 4868

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 0.05–0.5 µg/mL
  • Immunohistochemical techniques: 5 µg/mL
  • Immunoprecipitation: 1–2 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

Nephrosis 1, congenital, Finnish type; CNF, NPHN, NPHS1, renal glomerulus-specific cell adhesion receptor




 
 
Products
Cat. # Product Package Size Price # of Units Select
29070A Anti-Human Nephrin (C) Rabbit IgG Affinity Purify 10 ug $113.00 *
29070B Anti-Human Nephrin (C) Rabbit IgG Affinity Purify 100 ug $499.00
29050A Anti-Human Nephrin (N) Rabbit IgG Affinity Purify 10 ug $113.00 *
29050B Anti-Human Nephrin (N) Rabbit IgG Affinity Purify 100 ug $499.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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