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Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_E-G >  GBF1_Human

Anti-Human GBF1 Rabbit IgG Antibody

Brefeldin A is a fungal antibiotic that interferes with protein transport from the endoplasmic reticulum to the Golgi complex. Golgi brefeldin A-resistant guanine nucleotide exchange factor 1 (GBF1) regulates the recruitment of proteins to membranes by mediating the GDP to GTP exchange pathway. It is a member of the Sec7 domain family, and confers resistance to the action of brefeldin A It activates ADP ribosylation factor 1 (ARF1), thus blocking the first step in brefeldin A-mediated inhibition of GDP-GTP exchange. Phosphorylation of GBF1 at Thr(1337) by AMP-activated protein kinase has been shown to attenuate GBF1 activity. GBF1 is also known as BFA-resistant GEF 1, Golgi-specific brefeldin A resistance factor 1, ARF1GEF, and KIAA0248.

These products are affinity-purified IgG antibodies that recognize human GBF1 protein. The antibodies were raised in rabbit using synthetic peptides, and can be used for Western blot (WB) detection or immunoprecipitation (IP) of human GBF1 protein.

  At-A-Glance   Documents

Features

  • Cat.#: 28063A, 28063B
  • Target Name: Human GBF1 protein
  • Antigen: Human GBF1 C-terminal synthetic peptide
  • Application: WB, IP
  • Target Entrez Gene ID: 8729

  • Cat.#: 28065A, 28065B
  • Target Name: Human GBF1 protein
  • Antigen: Human GBF1 T1337-phosphorylated synthetic peptide
  • Application: WB, IP
  • Target Entrez Gene ID: 8729

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically):

  • Western blot: 1–5 µg/mL
  • Immunoprecipitation: 1–5 µg per reaction

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in 1.0 mL H2O (final concentration 100 µg/mL).

Alternative names

BFA-resistant GEF 1, Golgi-specific brefeldin A resistance factor 1, ARF1GEF, KIAA0248




 
 
Products
Cat. # Product Contents Size Price Units Select
28063A Anti-Human GBF1 (1844) Rabbit IgG Affinity Purify 10 ug $113.00 *
28063B Anti-Human GBF1 (1844) Rabbit IgG Affinity Purify 100 ug $499.00
28065A Anti-Human GBF1 (T1337 Phosphorylated) Rabbit IgG Affinity Purify 10 ug $113.00 *
28065B Anti-Human GBF1 (T1337 Phosphorylated) Rabbit IgG Affinity Purify 100 ug $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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