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Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_A_D >  Cre_Recombinase

Cre Recombinase Antibody

The Cre Recombinase Antibody is an affinity-purified rabbit IgG antibody that recognizes the middle portion of Cre recombinase enzyme. The Cre Recombinase antibody is raised in rabbit using a synthetic peptide and can be applied to Western blot (WB) or immunohistochemical (IHC) detection of Cre recombinase.

Cre recombinase is a 38-kDa enzyme derived from bacteriophage P1 and is a member of the integrase family of site-specific recombinases. It is also known as Cre cyclization recombinase. It uses a topoisomerase I-like mechanism to catalyze a site-specific recombination event between two DNA recognition sites known as loxP sites (two 13-bp inverted repeats separated by an 8-bp asymmetric spacer region). Cre recombinase plays important roles in the life cycle of bacteriophage P1, including cyclization of the linear genome and resolution of dimeric chromosomes that form after DNA replication. Cre recombinase is a widely used tool in molecular biology for manipulating DNA; for example, during gene knock-out or knock-in studies1.

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Features

Cat.# 18771A, 18771B
  • Name: Cre recombinase
  • Antigen: Cre recombinase (middle portion) synthetic peptide
  • Application: WB, IHC
  • Entrez Gene ID: 2777477

Applications

Recommended antibody concentration (optimal concentration should be determined empirically):

  • WB: 1–3 µg/mL
  • IHC: 2–5 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute each 5 ug vial in 50 ul H2O and each 50 ug vial in 0.5 mL H2O (final concentration, 100 µg/mL).

Alternative names

Cre cyclization recombinase


References

1. Topaloglu, O. et al. (2005) Nucleic Acids Res. 33(18):e158.



 
 
Products
Cat. # Product Contents Size Price Units Select
18771A Anti-Cre recombinase Rabbit IgG Affinity Purify 5 ug $113.00 *
18771B Anti-Cre recombinase Rabbit IgG Affinity Purify 50 ug $499.00
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