The Cre Recombinase Antibody is an affinity-purified rabbit IgG antibody that recognizes the middle portion of Cre recombinase enzyme. The Cre Recombinase antibody is raised in rabbit using a synthetic peptide and can be applied to Western blot (WB) or immunohistochemical (IHC) detection of Cre recombinase.
Cre recombinase is a 38-kDa enzyme derived from bacteriophage P1 and is a member of the integrase family of site-specific recombinases. It is also known as Cre cyclization recombinase. It uses a topoisomerase I-like mechanism to catalyze a site-specific recombination event between two DNA recognition sites known as loxP sites (two 13-bp inverted repeats separated by an 8-bp asymmetric spacer region). Cre recombinase plays important roles in the life cycle of bacteriophage P1, including cyclization of the linear genome and resolution of dimeric chromosomes that form after DNA replication. Cre recombinase is a widely used tool in molecular biology for manipulating DNA; for example, during gene knock-out or knock-in studies1.