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Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_A_D >  CD20

Anti-CD20 Monoclonal Antibody

This product is an anti-CD20 mouse monoclonal antibody. The CD20 antibody was raised against the N-terminus of CD20 and can be used to detect human CD20 protein by Western blot or CD20 positive cells by immunohistochemistry. CD20 (encoded by MS4A1 in humans) is a B-cell-specific surface molecule that is expressed during most stages of B-cell development. The normal function of CD20 is not well-understood, although it is thought to play a role in B-cell development. High CD20 expression is characteristic of most B-cell malignancies, including non-Hodgkin lymphoma and chronic lymphocytic leukemia. CD20-targeted therapies for these cancers include Rituximab, a chimeric monoclonal antibody that recognizes CD20 and destroys B-cells.

It has been reported that C-terminal mutations of CD20 are related to Rituximab-resistance1. This antibody recognizes the N-terminal region of CD20 and can be used to detect CD20 protein regardless of C-terminal mutational status.

At-A-Glance Documents Images & Data

Features

Cat. # 10415A/B

  • Target Name: Human CD20 N-terminus synthetic peptide (CMQSGPKPLFRRMSS)
  • Antigen: Purified CD25
  • Clone: 4-6H:2C
  • Application: WB, IHC
  • Entrez Gene ID: 931

Applications

Recommended antibody concentration (the optimal concentration should be determined empirically)

  • Western blot: 1 µg/mL
  • IHC: For FFPE tissues after heat-induced antigen retrieval, 1 µg/mL

Storage

Lyophilized: stable 5 years at 2–8°C. In solution: stable 2 years at –20°C.

Form

Lyophilized from an antibody solution containing 1% BSA in PBS and 0.05% NaN3. To use, reconstitute in H2O (final concentration, 100 µg/mL).

Alternative Names

MS4A1, B1, S7, Bp35, CVID5, MS4A2, LEU-16

Reference

  1. Mishima, Y., et al. (2011) The identification of irreversible rituximab-resistant lymphoma caused by CD20 gene mutations. Blood Cancer J. 1(4):e15.

Notes

For research use only. Not for use in diagnostic procedures.




 
 
Products
Cat. # Product Package Size Price # of Units Select
10415A Anti-Human CD20 (N) (4-6H:2C) Mouse IgG MoAb 10 ug $100.00
10415B Anti-Human CD20 (N) (4-6H:2C) Mouse IgG MoAb 100 ug $525.00
 

 


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