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Products >  Cell_Biology >  Miscellaneous >  Primary_Antibodies_A_D >  Ago2-Human_Monoclonal

Human Ago2 N-terminal Monoclonal Antibody

Human Ago2 N-terminal monoclonal antibody, also known as argonaute 2 antibody, is generated against the N-terminal region of human recombinant protein Argonaute 2, a member of the Argonaute family of proteins. Argonaute 2 is a transcription factor that is required for RNA-mediated gene silencing or RNA interference (RNAi). Argonaute proteins bind small interfering RNA (siRNA) and exhibit endonuclease activity against RNA-RNA duplexes consisting of mRNA bound to its complementary siRNA fragment. This Ago2 antibody is suitable for use in Western blot analysis.

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Applications

  • Western blot analysis under reducing and non-reducing conditions.


 

Source

Human Ago2 N-terminal monoclonal antibody was obtained by fusing the the mouse myeloma cell line P3U1 with lymph cells of BALB/c mouse lymph cells following immunization with the N-terminal peptide (1 - 148) of recombinant human Argonaute 2 (Ago2) protein (NP_036286.2). The Argonaute 2 antibody was purified from mouse ascitic fluid.

Storage

4°C

This product does not contain preservative.

The stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for up to 1 year or at 4°C for up to 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw. Diluted antibody should not be stored.

Specificity:

Clone No.SpecificitySubclass
hAgo2 1B1Human Ago2 proteinMouse IgG2a (lambda)

Form

Lyophilized

Reconstitution:

Dissolve the lyophilized antibody in 100 μl of distilled water (final concentration: 2.0 mg/ml). This solution can be used as a stock solution. If further dilution is required, use the noted dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μl or more of the noted dilution solution.

Note (1) : Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability.

Note (2) : Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Dilution solution:

10 mM PBS (pH7.4)

1.0% BSA

(0.1% NaN3)*

* When stored at 4°C, 0.1% sodium azide should be added as a preservative.

Reference

1) Azuma-Mukai, A. et al. (2008) Proc. Natl. Acad. Sci. USA 5(23):79647969.




 
 
Products
Offers Cat. # Product Contents Size Price Units Select
M211 Anti-Human Ago2, Monoclonal (Clone 1B1) 0.2 mg $315.00
 

 

 
 

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