Features- Monoclonal antibody for high specificity
- Does not cross-react with other E. coli proteins or the ProS2 tag
Applications- Detection of Trigger Factor-tagged recombinant proteins expressed using the pCold TF vector
- Western blotting under reducing or non-reducing conditions
- Immunoprecipitation
Component0.1mg Mouse IgG1 (lyophilized)
SourceMonoclonal antibody was obtained by fusing the P3U1 myeloma cell line with lymph node cells of C57BL/6 mouse after immunization with synthetic peptide (165-178) ［TIDFTGSVDGEEFE] of Trigger Factor conjugated with KLH. The monoclonal antibody was harvested from ascitic fluid of SCID mouse. Antibody was purified by column chromatography, dissolved in 10 mM PBS, pH7.4,containing 1.0% bovine serum albumin, and then lyophilized. The tig gene was obtained from Escherichia coli str. K-12 substr. MG1655.
Storage4°C (lyophilized). Once reconstituted, the stock solution (2.0 mg/ml) should be stored in aliquots at -20°C for up to 1 year, or at 4°C for up to 6 months after adding 0.1% sodium azide. (Lyophilized antibody does not contain sodium azide.) Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.
ReconstitutionDissolve the lyophilized antibody in 50 μl of distilled water (final concentration 2.0 mg/ml). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with Dilution solution (composition shown below) just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μl or more of the Dilution solution. Notes:- Be sure to store the antibody at a minimum concentration of 2.0 mg/ml. A lower antibody concentration may result in decreased stability.
- Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Dilution solution- 10 mM PBS (pH7.4)
- 1.0％ BSA
- (0.1％ NaN3)*
* When stored at 4°C, 0.1% sodium azide should be added as a preservative.
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