Applications - Detection of ProS2-Fused Protein Expressed by Using pCold ProS2 DNA
- Western Blotting Analysis Under Reducing and Non-Reducing Conditions (0.5–1 µg/mL)
- Immunoprecipitation (10 µg/mL)
- Use with E. coli Cold Shock Expression System pCold ProS2 DNA
Source - Clone: ProS 7B-8F
- Immunogen: ProS2* tag protein
- Immunized Host: C57Bl6 mouse
- Subclass: IgG1
- Antibody titer: 1:2000
* ProS2 is about 23 kDa of a solubilization tag which is tandem-dimer of N-terminal domain of a soluble protein Protein S derived from myxobacteria Myxococcus xanthus. It is used at E. coli cold shock expression system pCold ProS2 DNA.
Storage 4°C. The stock solution (2.0 mg/mL) with Reconstitution Solution should be stored in aliquots at –20°C for 1 year, or should be stored at 4°C for 6 months. Avoid repeated freeze-thaw cycles. The diluted antibody should not be stored.
Properties - Form: Lyophilized
- Supplied Reconstitution Solution: 0.1% NaN3 (in water)
- Reconstitution: Dissolve the lyophilized antibody in 50 µL of Reconstitution Solution (final concentration: 2.0 mg/mL).
- Cross Reativity: This product does not react with proteins derived from E. coli, and TF (Trigger Factor) tag.
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