Applications - Analysis of the structure, function and metabolism of insulin
- Western blot analysis under non-reducing and non-heating conditions
- Immunohistochemistry of frozen tissue sections
SourceHuman insulin monoclonal antibody is generated by fusing the mouse myeloma cell line P3U1 with BALB/c mouse spleen cells following immunization with human insulin. The INS antibody is then purified from mouse ascitic fluid. | Clone | Immun. | Immun. host | Sub-
class | Antib. titer | | IS11-1 | human insulin | BALB/c mouse | IgG1 | 1:2,000 |
StorageLyophilized antibody is stable at 4°C for up to 2 years. The reconstituted solution can be stored at 4°C for up to 6 months or at –20°C for up to 1 year. Do not expose to repeated freeze-thaw.
NotesFor research use only. Not for clinical diagnostics.
Specificity
FormLyophilized ReconstitutionDissolve the lyophilized antibody in 100 µl of distilled water (final concentration: 2.0 mg/ml).
References1. Mancini, G. et al. (1965) Immunochemistry 2:235-254.
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