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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Soluble_alpha-Klotho_Human

Alpha-Klotho Assay for detection of alpha-Klotho Protein

The alpha-Klotho ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies to human alpha-Klotho protein. A mouse monoclonal Klotho antibody is precoated onto the Klotho ELISA plate; the other mouse monoclonal Klotho antibody is HRP-conjugated. The Klotho ELISA can be used to measure soluble human alpha-Klotho protein in human blood, serum and plasma.

The alpha-Klotho gene was originally identified in alpha-kl(-/-) genetically modified mice that show a phenotype closely associated with human aging, including symptoms of arteriosclerosis, osteoporosis, skin atrophy, pulmonary emphysema, depletion of Purkinje cells in the cerebellum and infertility after 3–4 weeks1. Because of this association, soluble alpha-Klotho protein is a useful parameter in aging studies and aging research.

Alpha-Klotho protein is a 130 kDa transmembrane protein involved in calcium homeostasis and vitamin D regulation. The protein is expressed primarily in the kidneys and parathyroid glands.

At-A-Glance Documents Images & Data Resources

Features

Cat. #: 27998A
  • Capable of measuring human alpha-Klotho protein
  • Sensitivity of 6.15 pg/mL

Applications

  • Measuring alpha-Klotho protein in human serum or EDTA-plasma

Components

Precoated plate: Anti-Human Klotho (67G3) Mouse IgG MoAb
Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human Klotho
(91F1) Mouse IgG MoAb Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant human soluble alpha-Klotho0.5 mL x 2
EIA buffer:30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 94–6000 pg/mL
  • Assay Sensitivity: 6.15 pg/mL
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Human alpha-Klotho
  • Test specimen type: Human serum or EDTA-plasma
  • Sample volume per well: 100 µL

Reference

1. Manya, H. et al. (2010) Geriatrics & Gerontol. Int. 10(S1):S80-S87.




 
 
Products
Cat. # Product Package Size Price # of Units Select
27998A Human Soluble alpha-Klotho Assay Kit - IBL 96-Well $750.00
 

 



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