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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Prorenin_Receptor_Soluble_Human

Soluble (Pro)renin Receptor ELISA

The Soluble (Pro)renin Receptor Assay Kit is a solid-phase sandwich ELISA that uses two highly specific antibodies that recognize (pro)renin receptor protein; one antibody is precoated onto the ELISA plate and the other is HRP-conjugated. The Soluble (Pro)renin ELISA can be used to measure human soluble (pro)renin receptor protein in cell culture supernatant, serum, EDTA-plasma, or urine. This kit can also be used to measure mouse and rat soluble (pro)renin receptor protein in cell culture supernatant, serum, or EDTA-plasma

The (pro)renin receptor is responsible for tissue uptake of circulating renin/prorenin. This transmembrane receptor binds prorenin with higher affinity than renin. Binding of renin/prorenin to the receptor activates a pathway that results in generation of angiotensin. Research into (pro)renin receptor may uncover new strategies for suppressing tissue RAA (Renin-Angiotensin-Aldosterone) system overactivation. Human (pro)renin receptor is also involved in angiotensin-independent pathways that contribute to myocardial fibrosis and deterioration of cardiac function.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human prorenin receptor protein
  • Sensitivity: 24 pg/mL

Applications

  • Measurement of human soluble (pro)renin receptor protein in serum, EDTA-plasma, and urine.
  • Measurement of mouse and rat soluble (pro)renin receptor protein in serum and EDTA-plasma.
  • Measurement of soluble (pro)renin receptor protein in cell culture supernatant.

Components

Precoated plate: Anti-(Pro)renin Receptor Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-(Pro)renin Receptor
(93A1B) Mouse IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human (Pro)renin Receptor0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution 15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 125–8000 pg/mL
  • Assay sensitivity: 24 pg/mL
  • Assay duration: Overnight
  • Specificity: Human, mouse, and rat (pro)renin receptor
  • Species cross-reactivity: bovine 
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27782A Soluble (Pro)renin Receptor Assay Kit - IBL 96-Well $849.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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