The Human Prorenin ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies to the human prorenin receptor; one antibody is precoated onto the Human Prorenin ELISA plate and the other is HRP-conjugated. The Human Prorenin ELISA can be used to measure soluble human prorenin receptor protein in blood or urine.
The human prorenin receptor is responsible for tissue uptake of circulating renin/prorenin. This receptor is a 350-amino acid protein with a single transmembrane domain and binds prorenin with higher affinity than renin. Binding of renin/prorenin to the receptor activates a pathway that results in generation of angiotensin. However, the human prorenin receptor is also involved in angiotensin-independent pathways that contribute to myocardial fibrosis and deterioration of cardiac function.