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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Leptin

Leptin ELISA Kits

Leptin is an adipose-derived hormone that was discovered in studies of obesity in mice. It acts through a signaling pathway to regulate energy (food) intake and expenditure, maintaining homeostasis of adipose tissue. In humans, leptin is a 16 kDa protein, encoded by the LEP gene. Leptin is also known as obesity protein (OB), obesity factor, OBS, and LEPD. It is expressed mostly in white adipose tissue and acts through the leptin receptor, which exists as multiple isoforms. In addition to its role in adipose tissue, leptin regulates immune and inflammatory responses, hematopoiesis, angiogenesis, and wound healing. Mutations in the LEP gene cause severe to morbid obesity and hypogonadism, and are associated with the development of type 2 diabetes.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to leptin; one is precoated on the ELISA plate and the other is HRP-conjugated. These assay kits can be used to measure soluble human, mouse, or rat leptin protein in serum, EDTA plasma, or cell culture supernatants.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human, rat, or mouse leptin
  • Sensitivity: 0.86 pg/mL (Cat.# 27160); 10.82 pg/mL (Cat.# 27295); 2.13 pg/mL (Cat.# 27775)

Applications

  • Measuring human, rat, or mouse leptin protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Leptin Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Leptin Rabbit IgG
Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human/Mouse or Native Rat Leptin0.5 mL x 2
EIA buffer : 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 12.5–800 pg/mL (Cat.# 27160); 56.25–3600 pg/mL (Cat.# 27295); 15.63–1000 pg/mL (Cat.# 27775)
  • Assay sensitivity: 0.86 pg/mL (Cat.# 27160); 10.82 pg/mL (Cat.# 27295); 2.13 pg/mL (Cat.# 27775)
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Cross-reactivities: rat 43.6%, human 0.3% (Cat.# 27160); mouse 17.9%, human 0.2% (Cat.# 27295); mouse 2.4%, rat ≤0.1% (Cat.# 27775)
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27775A Human Leptin (highly sensitive) Assay Kit - IBL 96-Well $575.00
27160A Mouse Leptin Assay Kit - IBL 96-Well $575.00
27295A Rat Leptin Assay Kit - IBL 96-Well $575.00
 

 



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