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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Human_HTGL_Assay_Kit

Human HTGL ELISA

Hepatic triglyceride lipase or HTGL is encoded by the LIPC gene and is expressed primarily in the liver. HTGL, also known as hepatic lipase, is a lipolytic enzyme expressed by hepatocytes and plays a role in lipoprotein metabolism as well as the hydrolysis of triglycerides (TGs), phospholipids, intermediate-density lipoproteins (IDLs), and high-density lipoproteins (HDLs). HTGL has a high affinity for heparin, and measurement of HTGL in plasma is typically performed after intravenous injection with heparin (post-heparin plasma).

This kit is a solid phase sandwich ELISA that uses two highly specific antibodies to detect human HTGL (LIPC); one is precoated on the ELISA plate, and the other is HRP-conjugated. This kit can be used to measure human HTGL in post-heparin plasma collected with EDTA in cell culture supernatant.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human HTGL (LIPC, Entrez Gene ID: 3990)
  • Sensitivity: 0.08 ng/mL

Applications

  • Measurement of human HTGL from post-heparin plasma collected with EDTA in cell culture supernatant

Components

                
Precoated plate: Anti-Human HTGL (31A1) Mouse IgG MoAb Affinity Purify96 Well x 1
Labeled antibody (30X): HRP conjugated Anti- Human HTGL (26A1)
Mouse IgG Fab’ Affinity Purify
0.4 mL x 1
Standard: Recombinant human HTGL0.5 mL x 2
EIA buffer30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): Phosphate buffer50 mL x 1

Storage

2 - 8°C

Performance

  • Range of assay: 0.47 - 30 ng/mL
  • Assay sensitivity: 0.08 ng/mL
  • Assay duration: overnight
  • Test specimen type: EDTA-plasma and cell culture supernatant
  • Sample volume per well: 100 µL

Notes

For research use only, not for use in diagnostic procedures.


 
Products
Cat. # Product Package Size Price # of Units Select
27183A Human HTGL Assay Kit - IBL 96-Well $930.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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