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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  GLP-1_Active_Form

GLP-1 ELISA

The GLP-1 ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies to the active forms of human Glucagon-Like Peptide-1 or GLP-1; one antibody is precoated onto the GLP-1 ELISA plate while the other is HRP-conjugated. This GLP-1 assay kit can be used to measure the active forms of human GLP-1 in EDTA-plasma.

The protein encoded by the human proglucagon (GCG) gene is processed after translation into several polypeptides, each of which undergoes further modification. Two of these polypeptides constitute the biologically active forms of glucagon-like polypeptide 1 (GLP-1): GLP-1-(7-37) and GLP-1-(7-36)NH2. These active forms have a short half-life in the blood and are rapidly degraded by dipeptidyl peptidase 4 (DPP-4). GLP-1 is produced mostly in the intestinal L cells. It is a potent stimulator of glucose-dependent insulin secretion and a suppressor of plasma glucagon levels. Additional roles for GLP-1 have been proposed, including inhibition of gastric cell motility and secretion, stimulation of pancreatic beta cell proliferation, and inhibition of pancreatic beta cell apoptosis.

At-A-Glance Documents Images & Data

Features

Cat.# 27784A

  • Capable of measuring active forms of human GLP-1
  • Sensitivity: 0.10 pmol/L

Applications

  • Measurement of human GLP-1 active forms in EDTA-plasma.

Components

Precoated plate: Anti-GLP-1 Rabbit IgG, affinity purified96 Well x 1
Labeled antibody (30X): HRP-conjugated Anti-GLP-1 (34B1) Mouse
IgG MoAb Fab, affinity purified
0.4 mL x 1
Standard: GLP-1 (7-36) amide0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 1.25–80 pmol/L
  • Assay sensitivity: 0.10 pmol/L
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Reacts with GLP-1-(7-36)NH2 and GLP-1-(7-37); shows 0.32% cross-reactivity with GLP-1-(1-37); shows <0.1% cross-reactivity with GLP-1-(9-36)NH2, GLP-2, glucagon, human gastric inhibitory polypeptide (GIP) and mouse GIP
  • Test specimen type: EDTA-plasma
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27784A GLP-1, Active form Assay Kit - IBL 96-Well $606.00 *
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