The GLP-1 ELISA is a solid-phase sandwich ELISA that uses two highly specific antibodies to the active forms of human Glucagon-Like Peptide-1 or GLP-1; one antibody is precoated onto the GLP-1 ELISA plate while the other is HRP-conjugated. This GLP-1 assay kit can be used to measure the active forms of human GLP-1 in EDTA-plasma.
The protein encoded by the human proglucagon (GCG) gene is processed after translation into several polypeptides, each of which undergoes further modification. Two of these polypeptides constitute the biologically active forms of glucagon-like polypeptide 1 (GLP-1): GLP-1-(7-37) and GLP-1-(7-36)NH2. These active forms have a short half-life in the blood and are rapidly degraded by dipeptidyl peptidase 4 (DPP-4). GLP-1 is produced mostly in the intestinal L cells. It is a potent stimulator of glucose-dependent insulin secretion and a suppressor of plasma glucagon levels. Additional roles for GLP-1 have been proposed, including inhibition of gastric cell motility and secretion, stimulation of pancreatic beta cell proliferation, and inhibition of pancreatic beta cell apoptosis.