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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  GLP-1

GLP-1 ELISA Kits, Active and Inactive Forms

The protein encoded by the human proglucagon (GCG) gene is processed after translation into several polypeptides, each of which undergoes further modification. Two of these polypeptides constitute the biologically active forms of glucagon-like polypeptide 1 (GLP-1): GLP-1-(7-36)NH2 and GLP-1-(7-37). These active forms have a short half-life in the blood and are rapidly degraded by dipeptidyl peptidase 4 (DPP-4) into the inactive forms: GLP-1-(9-36)NH2 and GLP-1-(9-37).

GLP-1 is produced mostly in the intestinal L cells. It is a potent stimulator of glucose-dependent insulin secretion and a suppressor of plasma glucagon levels. Additional roles for GLP-1 have been proposed, including inhibition of gastric cell motility and secretion, stimulation of pancreatic beta cell proliferation, and inhibition of pancreatic beta cell apoptosis. The GLP-1 ELISA kits are solid-phase sandwich ELISAs that use two highly specific antibodies to either the active or inactive forms of human GLP-1.

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Features

Cat.# 27784A

  • Capable of measuring active forms of human GLP-1 in human EDTA plasma. (We recommend using DPP-4 inhibitor when collecting blood.)
  • Sensitivity: 0.10 pmol/L

Cat.# 27788A

  • Capable of measuring inactive forms of GLP-1 in in human, mouse, and rat EDTA plasma and cell culture supernatant.
  • Sensitivity: 0.36 pmol/l

Applications

  • Measurement of active (Cat. # 27784A) or inactive (Cat. # 27788A) forms of GLP-1.

Components

Cat. # 27784A

Precoated plate: Anti-GLP-1 Rabbit IgG, affinity purified96 well x 1
Labeled antibody (30X): HRP-conjugated Anti-GLP-1 (34B1) Mouse IgG MoAb Fab, affinity purified0.4 ml x 1
Standard: GLP-1 (7-36) amide0.5 ml x 2
EIA buffer: 1% BSA, 0.05% Tween-20 in PBS30 ml x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween-20 in PBS12 ml x 1
Chromogen: TMB solution15 ml x 1
Stop solution: 1N H2SO412 ml x 1
Wash buffer (40X): 0.05% Tween-20 in phosphate buffer50 ml x 1

Cat. # 27788A

Precoated plate: Anti-GLP-1 (C) (19A1) Mouse IgG MoAb96 well x 1
Labeled antibody (30X): HRP-conjugated Anti-GLP-1 (59B2A) Mouse IgG MoAb Fab0.4 ml x 1
Standard: GLP-1, inactive form0.5 ml x 2
EIA buffer: 1% BSA, 0.05% Tween-20 in PBS30 ml x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween-20 in PBS12 ml x 1
Chromogen: TMB solution15 ml x 1
Stop solution: 1N H2SO412 ml x 1
Wash buffer (40X): Phosphate buffer50 ml x 1

Storage

2–8°C

Performance

Cat. # 27784A

  • Range of assay: 1.25–80 pmol/l
  • Assay sensitivity: 0.10 pmol/l
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Reacts with GLP-1-(7-36)NH2 and GLP-1-(7-37); shows 0.32% cross-reactivity with GLP-1-(1-37); shows <0.1% cross-reactivity with GLP-1-(9-36)NH2, GLP-2, glucagon, human gastric inhibitory polypeptide (GIP) and mouse GIP
  • Test specimen type: EDTA-plasma
  • Sample volume per well: 100 µL

Cat. # 27788A

  • Range of assay: 1.25–80 pmol/l
  • Assay sensitivity: 0.36 pmol/l
  • Assay duration: Approximately 3–3.5 hours
  • Specificity: Reacts with GLP-1, Inactive form; shows 0.3% cross-reactivity with GLP-1, Active form
  • Test specimen type: EDTA plasma and cell culture supernatant
  • Sample volume per well: 100 µl



 
 
Products
New Offers Cat. # Product Contents Size Price Units Select
  27784A GLP-1, Active form Assay Kit - IBL 96-Well $606.00 *
27788A GLP-1, Inactive form Assay Kit - IBL 96-Well $892.00
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 
 

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