Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Cell Biology


Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  GIP_Active_Form

Gastric Inhibitory Polypeptide (GIP) ELISA

The Gastric Inhibitory Polypeptide (GIP) ELISA kits are solid-phase ELISAs that use two highly specific GIP antibodies; one GIP antibody is precoated onto the ELISA plate and the other antibody is HRP-conjugated. These GIP assay kits can measure soluble active GIP (1-42) or total GIP (active (1-42) and inactive (3-42)) in EDTA-plasma. Please note that GIP (1-42) is rapidly inactivated by dipeptidyl peptidase IV (DPP-IV); therefore, samples should be treated with a DPP-IV inhibitor before performing assays for the active form.

Gastric inhibitory polypeptide (GIP), also known as glucose-dependent insulinotropic peptide, is a member of the incretin family of hormones. Following food ingestion, these gastrointestinal hormones promote insulin secretion from beta cells while inhibiting glucagon release from alpha cells within the islets of Langerhans. In addition to the pancreas, GIP receptor expression has been reported in adipocytes and osteoblasts. The regulation of GIP signaling pathways may lead to metabolic syndrome improvement. GIP is derived from a 153-amino acid precursor encoded by the GIP gene. The biologically active form of GIP is a 42-amino acid peptide termed GIP (1-42). GIP is rapidly inactivated to GIP (3-42) by blood DPP-IV.

  At-A-Glance   Documents   Images & Data

Features

  • Capable of quantifying human, rat, or mouse GIP protein

Applications

  • For measurement of active GIP (1-42) protein from human, rat, or mouse EDTA-plasma.
  • For measurement of total GIP from human, rat, or mouse EDTA-plasma.

Components

Precoated plate: Anti-GIP(C) Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-GIP (N) (6A1A) Mouse
IgG Fab Affinity-Purified
0.4 mL x 1
Standard: GIP(1-42)0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB Solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

Cat. #27764A27201A27202A27203A27204A27205A
Range of assay7.8–500 pg/mL 1.6–100 pg/mL2.3–150 pg/mL1.88 - 120 pmol/L 12.5 - 800 pmol/L2.34 - 150 pmol/L
Assay sensitivity 1.5 pg/mL1.2 pg/mL 0.43 pg/mL0.82 pmol/L4.1 pmol/L0.8 pmol/L
Specificity Active mouse GIPActive human GIPActive rat GIPTotal human GIPTotal mouse GIPTotal rat GIP
     
  • Assay duration: Approximately 3–3.5 hours
  • Test specimen type: EDTA-plasma
  • Sample volume per well: 100 µL

 
Products
New Cat. # Product Contents Size Price Units Select
  27764A Mouse GIP, Active form Assay Kit - IBL 96-Well $849.00 *
  27201A Human GIP, Active form Assay Kit - IBL 96-Well $849.00 *
  27202A Rat GIP, Active form Assay Kit - IBL 96-Well $849.00 *
  27203A Human GIP, Total Assay Kit - IBL 96-Well $930.00 *
27204A Mouse GIP, Total Assay Kit - IBL 96-Well $930.00 *
27205A Rat GIP, Total Assay Kit - IBL 96-Well $930.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions