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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Endothelin-3

Endothelin-3 ELISA Kit

Endothelins are among the most potent vasoconstrictor proteins known. Overexpression of endothelins contributes to hypertension and heart disease. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2), and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Recently, 31-amino acid endothelins were discovered. These ET(1-31) proteins are generated by specific cleavage of big endothelin by human chymase. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary, and placenta. Endothelin-3 is involved in the development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Endothelin-3 is also known as PPET3, HSCR4, and WS4B.

This kit is a solid-phase sandwich ELISA using two antibodies that are specific to human ET-3 protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This kit can be used to measure soluble ET-3 protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts.

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Features

  • Capable of measuring human ET-3
  • Sensitivity of 0.36 pg/mL

Applications

  • Measuring ET-3 protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts

Components

Precoated plate: Anti-ET-315-21 Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-ET-3 Rabbit IgG
Fab Affinity-Purified
0.4 mL x 1
Standard: ET-3 Peptide0.5 mL x 2
EIA buffer : 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution 15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.78–50 pg/mL
  • Assay sensitivity: 0.36 pg/mL
  • Assay duration: Overnight
  • Specificity: Reacts with human ET-3; 8% cross-reactivity with ET-1; <0.1% cross-reactivity with ET (1-31) or big ET isoforms
  • Species cross-reactivity: 0.6% with rat big ET-1
  • Test specimen type: Serum, EDTA-plasma, cell culture supernatants, or tissue extracts
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27169A Endothelin-3 Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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