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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Endothelin-1

Endothelin-1 ELISA Kit

Endothelins are among the most potent vasoconstrictor proteins known. Overexpression of endothelins contributes to hypertension and heart disease. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2), and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Recently, 31-amino acid endothelins were discovered. These ET(1-31) proteins are generated by specific cleavage of big endothelin by human chymase. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary and placenta.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to ET-1 protein or ET-1(1-31); one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble ET-1 or ET-1(1-31) protein in serum, EDTA-plasma, cell culture supernatants or tissue extracts.

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Features

  • Capable of measuring human, bovine, canine, murine, porcine, rabbit, and rat ET-1 or ET-1(1-31)
  • Sensitivity of 0.23 pg/mL (Cat.# 27165); 0.62 pg/mL (Cat.# 27177)

Applications

  • Measuring ET-1 or ET-1(1-31) protein in serum, EDTA-plasma, cell culture supernatants or tissue extracts

Components

Precoated plate: Anti-ET-1 Rabbit IgG Affinity-Purified96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-ET-1
Rabbit IgG Fab Affinity-Purified
0.4 mL x 1
Standard: ET-1 Peptide0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 0.78–100 pg/mL (Cat.# 27165); 1.56–200 pg/mL (Cat.# 27177)
  • Assay sensitivity: 0.23 pg/mL (Cat.# 27165); 0.62 pg/mL (Cat.# 27177)
  • Assay duration: Overnight
  • Specificity: Human, bovine, canine, murine, porcine, rabbit and rat ET-1; ≤0.1% cross-reactivity with ET-3; ≤0.1% (Cat.# 27165) or 0.6% (Cat.# 27177) cross-reactivity with big ET-1
  • Species cross-reactivity: Human, bovine, canine, murine, porcine, rabbit and rat
  • Test specimen type: Serum, EDTA-plasma, cell culture supernatants or tissue extracts
  • Sample volume per well: 100 µL

 
Products
Offers Cat. # Product Contents Size Price Units Select
27165A Endothelin-1 Assay Kit - IBL 96-Well $606.00 *
27177A Endothelin-1 (1-31) Assay Kit - IBL 96-Well $606.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 

 

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