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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Endothelin-1

Endothelin-1 ELISA Kit

Endothelins are among the most potent vasoconstrictor proteins known. Overexpression of endothelins contributes to hypertension and heart disease. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2), and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Recently, 31-amino acid endothelins were discovered. These ET(1–31) proteins are generated by specific cleavage of big endothelin by human chymase. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary and placenta.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to ET-1(1–21) protein or ET-1(1–31); one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble ET-1(1–21) or ET-1(1–31) protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts.

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Features

  • Capable of measuring human, bovine, canine, murine, porcine, rabbit, and rat ET-1(1–21) (Cat.# 17165A) or ET-1(1-31) (Cat.# 27177A)
  • Sensitivity of 0.39 pg/ml (Cat.# 17165A); 0.62 pg/ml (Cat.# 27177A)

Applications

  • Measuring ET-1(1–21) (Cat.# 17165A) or ET-1(1-31) (Cat.# 27177A) protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts

Components

Precoated plate: Anti-ET-1 Rabbit IgG Affinity-Purified      96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-ET-1
Rabbit IgG Fab Affinity-Purified
0.4 ml x 1
Standard: ET-1 Peptide0.5 ml x 2
EIA buffer30 ml x 1
Solution for labeled antibody12 ml x 1
Chromogen: TMB solution15 ml x 1
Stop solution12 ml x 1
Wash buffer (40X)50 ml x 1

Storage

2–8°C (Cat. # 27177A); –20°C (Cat. # 17165A)

Performance

  • Range of assay: 0.78–100 pg/ml (Cat.# 17165A); 1.56–200 pg/ml (Cat.# 27177A)
  • Assay sensitivity: 0.39 pg/ml (Cat.# 17165A); 0.62 pg/ml (Cat.# 27177A)
  • Assay duration: Overnight
  • Specificity: Human, bovine, canine, murine, porcine, rabbit, and rat ET-1; ≤0.1% cross-reactivity with ET-3; ≤0.1% (Cat.# 17165A) or 0.6% (Cat.# 27177A) cross-reactivity with big ET-1
  • Species cross-reactivity: Human, bovine, canine, murine, porcine, rabbit, and rat
  • Test specimen type: Serum, EDTA-plasma, cell culture supernatants, or tissue extracts
  • Sample volume per well: 100 µl



 
 
Products
New Cat. # Product Contents Size Price Units Select
  27177A Endothelin-1 (1-31) Assay Kit - IBL 96-Well $606.00
17165A Endothelin-1 Assay Kit - IBL 96-Well $697.00
 

 

 
 

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