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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Endothelin-1

Endothelin Detection

Endothelins are among the most potent vasoconstrictor proteins known. Overexpression of endothelins contributes to hypertension and heart disease. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2), and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Recently, 31-amino acid endothelins were discovered. These ET (1-31) proteins are generated by specific cleavage of big endothelin by human chymase. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary, and placenta. Overexpression of endothelins contributes to hypertension and heart disease. Endothelins mediate their activity through binding to two distinct endothelin receptors, known as endothelin A receptor and endothelin B receptor.

ET-1 is produced by vascular endothelial cells. Overexpression of ET-1 contributes to hypertension and heart disease, and also has effects on the central nervous system. ET-1 is also known as PPET1 and HDLCQ7.

The processed form of ET-2 binds to several endothelin receptors that initiate intracellular signaling events and control a range of processes, including hypertension and ovulation. ET-2 is also known as PPET2.

The active form of ET-3 is a ligand for endothelin receptor type B (EDNRB). This interaction is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in the ET-3 gene are associated with the congenital disorders Hirschsprung disease (HSCR) and Waardenburg syndrome (WS). Endothelin-3 is also known as PPET3, HSCR4, and WS4B.

The endothelin A receptor, encoded by the EDNRA gene, associates with guanine-nucleotide-binding (G) proteins; this interaction activates a phosphatidylinositol-calcium second messenger system. Endothelin A receptor binds ET-1 with high affinity and ET-2 with moderate affinity. It binds very weakly to ET-3. Endothelin A receptor is expressed in a variety of tissues, including lung, aorta, atrium, liver, muscle, cerebral cortex, cerebellum, kidney, adrenal, duodenum, colon, and placenta. Endothelin A receptor is also known as endothelin receptor type A, ET-A, ETA-R, ETRA, hET-AR, and ET-1 receptor.

The endothelin B receptor, encoded by the EDNRB gene, associates with guanine-nucleotide-binding (G) proteins; this interaction activates a phosphatidylinositol-calcium second messenger system. Endothelin B receptor binds ET-1 and ET-3 with high affinity. It is expressed in endothelial cells lining the wall of all blood vessels, and in lung, brain, and heart. Mutations in the EDRNB gene are linked to Waardenburg syndrome and Hirschsprung disease type 2. The endothelin B receptor is also known as endothelin receptor type B, ET-B, ETBR, ETRB, hET-BR, HSCR, HSCR2, ABCDS, and endothelin receptor nonselective type.

ELISAs for Endothelin Detection

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to ET-1(1-21), ET-1 (1-31), human ET-2 (1-31), or human ET-3 protein; one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble ET-1 (1-21), ET-1 (1-31), human ET-2 (1-31), or human ET-3 protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts.

Antibodies for Endothelin Detection

This suite of affinity-purified IgG antibody products recognizes the following: human endothelin A or B receptors; human, bovine, canine, mouse, porcine, and rat ET-1; human, mouse, and rat ET-3; or human ET-1, ET-2, and ET-3. The antibodies were raised in rabbit or mouse using a synthetic peptide or recombinant protein and can be used for Western blot (WB) detection or immunohistochemical (IHC) detection.

  At-A-Glance   Documents   Images & Data

Features

Antigen
Cat. #
Application
Species
(Clone) and Source
 
Cross‑Reactivity
 
ET-1 (1-31)27177AELISAHuman
Mouse Rat Rabbit Bovine Canine Porcine

Capture:
(n/a)
residues 25-31
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

Cross‑reacts with ≤0.1% cross-reactivity with ET‑1, ET‑3, and ET‑2 (1‑31)

0.6% cross-reactivity with human and rat big ET-1

ET-1 (1-21)17165AELISAHuman Mouse Rat Rabbit Bovine Canine Porcine

Capture:
(n/a)
Residues 15-21
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

3.32% cross‑reactivity with ET‑2 (1‑21)

≤0.16% cross-reactivity with
ET-3 (1-21), ET-1 (1-31), ET-2 (1-31), ET-3 (1-31), big ET-1, -2, -3, and VIC

ET-2 (1-31)17178AELISAHuman

Capture:
(n/a) residues
25-31 Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

8% cross-reactivity with big
ET-2; 1% cross-reactivity with ET-1 (1-31); ~0.2% cross-reactivity with human and rat big ET-1; <0.1% cross-reactivity with ET-3 (1-31), ET (1-21) isoforms, big ET-3, and VIC
ET-327169AELISAHumanCapture:
(n/a) residues 15‑21
Rabbit IgG

Detect: HRP
(n/a)
Rabbit IgG

7.5% cross-reactivity with ET-1; <0.1% cross-reactivity with ET-1 (1-31), ET-2 (1-31), and big ET-1

0.6% cross-reactivity with rat big ET-1

ET-A Receptor (C-terminus)16201A, BIHC
WB
Human(A405)
Rabbit IgG
Cross-reacts with bovine and rat ET-A receptor
ET-B Receptor10253A, BIHC
WB
Human(8Z11)
Mouse IgG2a MoAb
n/a
ET-B Receptor (N-terminus)16205BWBHuman(B27)
Rabbit IgG
Cross-reacts with bovine and rat ET-B receptor
ET-118201AIHCHuman Mouse Rat Bovine Canine Porcine(n/a)
Rabbit IgG
Cross-reacts with big ET-1; shows 0.2%–1.0% cross-reactivity with ET-2, ET-3, big ET-2, big ET-3, and VIC
ET-318202A, BIHC
WB
Human(n/a)
Rabbit IgG

Cross-reacts with big ET-3; shows 0.8% cross-reactivity with ET-1, ET-2, big ET-1, and big ET-2 as well as 0.1% cross-reactivity with VIC

Cross-reacts with mouse and rat ET-3

ET (15‑21) (C‑terminus)18204AIHCHuman(n/a)
Rabbit IgG

Cross-reacts with human ET-1, ET-2, ET-3, and VIC

<0.1% cross-reactivity with big ET-1, -2, and -3

Additional Information

Please see a product's User Manual and Data Sheet for information about storage conditions, product components, and technical specifications. Please see the User Manual to determine kit components. User Manuals and Data Sheets are located under the Documents tab.




 
 
Products
New Cat. # Product Contents Size Price Units Select
  27177A Endothelin-1 (1-31) Assay Kit - IBL 96-Well $606.00
17165A Endothelin-1 Assay Kit - IBL 96-Well $697.00
 

 

 
 

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