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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_E-Z >  Endothelial_Lipase

Human Endothelial Lipase (EL) ELISA Kit

Endothelial lipase (EL), encoded by the LIPG gene in humans, is a phospholipase that is involved in lipoprotein metabolism and vascular biology. EL is expressed at high levels in the liver, placenta, lung, thyroid, kidney, testis, and in the corpus luteum of the ovary. It hydrolyzes high-density lipoproteins (HDL) with high efficiency compared to very low-density lipoproteins (VLDL) and chylomicrons. It also displays triglyceride lipase and heparin-binding activities. EL is also known as EDL, endothelial cell-derived lipase, lipoprotein lipase H, and PRO719.

This kit is a solid-phase sandwich ELISA using two antibodies that are highly specific to human EL protein; one is precoated on the ELISA plate and the other is HRP-conjugated. This assay kit can be used to measure soluble human EL protein in serum, EDTA plasma, or cell culture supernatants.

At-A-Glance Documents

Features

  • Capable of measuring human EL protein
  • Sensitivity: 5.7 pg/mL

Applications

  • Measuring human EL protein in serum, EDTA plasma, or cell culture supernatants

Components

Precoated plate: Anti-Human EL (48A1A) Mouse
IgG MoAb Affinity-Purified
96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-Human EL
(26A1A) Mouse IgG Fab Affinity-Purified
0.4 mL x 1
Standard: Recombinant Human Endothelial Lipase0.5 mL x 2
EIA buffer:30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS12 mL x 1
Chromogen: TMB solution15 mL x 1
Stop solution:  1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance

  • Range of assay: 31.3–2000 pg/mL
  • Assay sensitivity: 5.7 pg/mL
  • Assay duration: Overnight
  • Test specimen type: Serum, EDTA plasma, or cell culture supernatants
  • Sample volume per well: 100 µL

 
Products
Cat. # Product Package Size Price # of Units Select
27182A Human Endothelial Lipase (EL) Assay Kit - IBL 1 x 96-Well Plate $930.00 *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 

 


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