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Products >  Cell_Biology >  Metabolic_Diseases >  ELISA_Kits_A-D >  Big_Endothelin-1_ELISA

Big Endothelin-1 ELISA Kits

Endothelins are among the most potent vasoconstrictor proteins known. Overexpression of endothelins contributes to hypertension and heart disease. Three isoforms are known, each containing 21 amino acids: endothelin-1 (ET-1), endothelin-2 (ET-2) and endothelin-3 (ET-3). All three isoforms are encoded by a 38-amino-acid precursor known as big endothelin. Endothelins are expressed in many tissues, including lung, kidney, brain, pituitary and placenta.

These kits are solid-phase sandwich ELISAs using two antibodies that are highly specific to human or rat big ET-1 protein; one is precoated on the ELISA plate, and the other is HRP-conjugated. These kits can be used to measure soluble human or rat big ET-1 protein in serum, EDTA-plasma, cell culture supernatants or tissue extracts.

At-A-Glance Documents Images & Data

Features

  • Capable of measuring human or rat big ET-1
  • Sensitivity of 0.69 pg/mL (Cat.# 27167); 0.30 pg/mL (Cat.# 27168)

Applications

  • Measuring big ET-1 protein in serum, EDTA-plasma, cell culture supernatants, or tissue extracts

Components

Precoated plate: Anti-big ET22-38 Rabbit IgG Affinity Purify96 Well x 1
Labeled antibody (30X): HRP conjugated Anti-ET-1 Rabbit IgG
Fab Affinity-Purified
0.4 mL x 1
Standard: Human or Rat Big ET-1 (Peptide)0.5 mL x 2
EIA buffer: 1% BSA, 0.05% Tween 20 in PBS30 mL x 1
Solution for labeled antibody: 1% BSA, 0.05% Tween 20 in PBS 12 mL x 1
Chromogen: TMB solution 15 mL x 1
Stop solution: 1N H2SO412 mL x 1
Wash buffer (40X): 0.05% Tween 20 in phosphate buffer50 mL x 1

Storage

2–8°C

Performance:

  • Range of assay: 0.78–100 pg/mL
  • Assay sensitivity: 0.69 pg/mL (Cat.# 27167); 0.30 pg/mL (Cat.# 27168)
  • Assay duration: Overnight
  • Specificity: Human, mouse/rat big ET-1; ≤ 0.1% against ET-1, ET-3
  • Species cross-reactivity: Human, rat
  • Test specimen type: Serum, EDTA-plasma, cell culture supernatants or tissue extracts
  • Sample volume per well: 100 µL



 
 
Products
Cat. # Product Package Size Price # of Units Select
27167A Rat Big Endothelin-1 Assay Kit - IBL 96-Well $575.00
27168A Human Big Endothelin-1 Assay Kit - IBL 96-Well $575.00
 

 



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